| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Outline of Final Research Achievements |
Electn transfer dissociation (ETD) tandem mass spectrometry have been used as analytical method for characterization of protein. Since ECD/ETD involved the recombination between peptide ion and electron/anion, employing ions with a higher charge state as the precursor dramatically increases the yield of radical reactive species and thereby improving the sequence coverage obtained with ETD. Hoerver, the charge state of peptides is dependent on the peptide sequence, especially on the number of basic residues. For analysis of tryptic peptides, the N-terminal amino group and the C-terminal basic residue are protonated, often yielding doubly protonated species. However, double protonation is not enough for the ion/electron reaction of ETD to yield better sequence coverage. We found that the use of a suitable metal salt in analyte solution would increase the charge state of tryptic peptide ions containing acidic residues and provide information useful for sequencing by ETD.
|
