Use of zebrafish as a model animal for gerontology

• Project/Area Number: 26520101
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 特設分野
• Research Field: Neo-Gerontology
• Research Institution: University of Tsukuba
• Principal Investigator: Kobayashi Makoto 筑波大学, 医学医療系, 講師 (50254941)
• Project Period (FY): 2014-07-18 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: ゼブラフィッシュ / 老年度 / 運動量 / 聴力 / 学習力 / Nrf2経路 / 食品ファイトケミカル / 運動力 / モデル動物 / 集団飼育 / ストレス / 発がん / 老化 / Nrf2システム

Outline of Final Research Achievements

We investigated a usefulness of zebrafish for understanding effects of lifestyle and genetic background on aging. As a result, 1) we succeeded in setting up the evaluation system of zebrafish aging degree , and showed that swimming and hearing abilities were lower in aged fish than young fish; 2) we found that there is a tendency that old male fish was healthy when we bred it together with young females; 3) to elucidate the relationship between aging and the Nrf2 pathway, a cellular master antioxidant system, we compared aging degrees of wild-type and Nrf2-mutant zebrafish, and showed that there was no clear difference between these two at the middle age stage; 4) we demonstrated that zebrafish is a good system to evaluate the effects of food phytochemicals. From these results, we conclude that zebrafish will be a useful model for gerontology, while more larger-scale and longer-term studies will be required to obtain meaningful data.

Academic Significance and Societal Importance of the Research Achievements

集団飼育と行動学的表現型解析のし易さ、さらには飲食品関連物質の機能性解析のし易さを示せたことから、学術的意義は、ゼブラフィッシュの老年学研究モデルとしての有効性、特に健康や老化における遺伝子機能の解析に優れた解析系となることを示唆した点である。一方、ヒト健康への応用に向けた説得力のある有意なデータを示すことができず、社会的意義という観点からは、解析数を増やすなどより大規模な研究を行う必要があることとヒト解析と連動させた研究を行う必要があることが分かり、今後の研究に向けての課題となった。

Research Products

• [Journal Article] Hdac1 Regulates Differentiation of Bipotent Liver Progenitor Cells During Regeneration via Sox9b and Cdk8 (2019)
  • Author(s): Ko Sungjin、Russell Jacquelyn O.、Tian Jianmin、Gao Ce、Kobayashi Makoto、Feng Rilu、Yuan Xiaodong、Shao Chen、Ding Huiguo、Poddar Minakshi、Singh Sucha、Locker Joseph、Weng Hong-Lei、Monga Satdarshan P.、Shin Donghun
  • Journal Title: Gastroenterology Volume: 156 Issue: 1 Pages: 187-202.e14
  • DOI: 10.1053/j.gastro.2018.09.039
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Nrf2 activation attenuates genetic endoplasmic reticulum stress induced by a mutation in the phosphomannomutase 2 gene in zebrafish (2018)
  • Author(s): Mukaigasa Katsuki、Tsujita Tadayuki、Nguyen Vu Thanh、Li Li、Yagi Hirokazu、Fuse Yuji、Nakajima-Takagi Yaeko、Kato Koichi、Yamamoto Masayuki、Kobayashi Makoto
  • Journal Title: Proc. Natl. Acad. Sci. Volume: 115 Issue: 11 Pages: 2758-2763
  • DOI: 10.1073/pnas.1714056115
  • Peer Reviewed / Open Access
• [Journal Article] The possible repositioning of an oral anti-arthritic drug, auranofin, for Nrf2-activating therapy: The demonstration of Nrf2-dependent anti-oxidative action using a zebrafish model. (2018)
  • Author(s): Fuse Y,Endo Y, Araoi S, Daitoku H, Suzuki H, Kato M, Kobayashi M.
  • Journal Title: Free Radic Biol Med Volume: 115 Pages: 405-411
  • DOI: 10.1016/j.freeradbiomed.2017.12.022
  • Peer Reviewed
• [Journal Article] MafB is a critical regulator of complement component C1q (2017)
  • Author(s): Tran, M.T.N., Hamada, M., Jeon, H.J., Shiraishi, R., Asano, K., Hattori, M., Nakamura, M., Imamura, Y., Tsunakawa, Y., Fujii, R., Kulathunga, K., Andrea, C.S., Koshida, R., Kamei, R., Matsunaga, Y., Kobayashi, M., Oishi, H., Kudo, T. and Takahashi, S.
  • Journal Title: Nature Commun. Volume: 8 Issue: 1 Pages: 1700-1700
  • DOI: 10.1038/s41467-017-01711-0
  • NAID: 120007128749
  • Peer Reviewed / Open Access
• [Journal Article] Conservation of the Keap1-Nrf2 system: An Evolutionary Journey through Stressful Space and Time. (2017)
  • Author(s): Fuse, Y. and Kobayashi, M.
  • Journal Title: Molecules Volume: 22 Issue: 3 Pages: 436-436
  • DOI: 10.3390/molecules22030436
  • NAID: 120007134369
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] 国内の研究者・技術者によるグラフデザインの実態 (2017)
  • Author(s): 田中 佐代子、小林 麻己人、三輪 佳宏
  • Journal Title: デザイン学研究 Volume: 63 Pages: 47-56
  • NAID: 130006907439
  • Peer Reviewed
• [Journal Article] 研究者のためのビジュアルデザイン ~「ビジュアルデザインハンドブック」の有用性 ~ (2017)
  • Author(s): 田中 佐代子、小林 麻己人、三輪 佳宏
  • Journal Title: 科学技術コミュニケーション Volume: 21 Pages: 41-57
  • NAID: 120006313477
  • Peer Reviewed
• [Journal Article] Nrf2-dependent protection against acute sodium arsenite toxicity in zebrafish. (2016)
  • Author(s): Fuse, Y., Nguyen, V.T. and Kobayashi, M.
  • Journal Title: Toxicol. Appl. Pharmacol. Volume: 305 Pages: 136-142
  • DOI: 10.1016/j.taap.2016.06.012
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Conservation of the Nrf2-mediated gene regulation of proteasome subunits and glucose metabolism in zebrafish. (2016)
  • Author(s): Nguyen, T.V., Fuse, Y., Tamaoki, J., Akiyama, S., Muratani, M., Tamaru, Y. and Kobayashi, M.
  • Journal Title: Oxid. Med. Cell. Longev. Volume: 2016 Issue: 1 Pages: 5720574-5720574
  • DOI: 10.1155/2016/5720574
  • NAID: 120007135115
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Evaluation of the toxicity and antioxidant activity of redox nanoparticles in zebrafish (Danio rerio) embryos. (2016)
  • Author(s): Vong, L.B., Kobayashi, M. and Nagasaki, Y.
  • Journal Title: Mol. Pharm. Volume: 13 Issue: 9 Pages: 3091-3097
  • DOI: 10.1021/acs.molpharmaceut.6b00225
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Sensory systems and ionocytes are targets for silver nanoparticle effects in fish. (2016)
  • Author(s): Osborne, O.J., Mukaigasa, K., Nakajima, H., Stolpe, B., Romer, I., Philips, U., Lynch, I., Mourabit, S., Hirose, S., Lead, J.R., Kobayashi, M., Kudoh, T. and Tyler, C.R.
  • Journal Title: Nanotoxicology Volume: 10 Issue: 9 Pages: 1276-1286
  • DOI: 10.1080/17435390.2016.1206147
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] LSD1/KDM1A promotes hematopoietic commitment of hemangioblasts through downregulation of Etv2. (2015)
  • Author(s): Takeuchi, M., Fuse, Y., Watanabe, M., Andrea, C.S., Takeuchi, M., Nakajima, H., Ohashi, K., Kaneko, H., Kobayashi-Osaki, M., Yamamoto, M. and Kobayashi, M.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 112 Issue: 45 Pages: 13922-13927
  • DOI: 10.1073/pnas.1517326112
  • NAID: 120007135765
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Heme-mediated inhibition of Bach1 regulates the liver specificity and transience of the Nrf2-dependent induction of zebrafish heme oxygenase 1. (2015)
  • Author(s): Fuse, Y., Nakajima, H., Nakajima-Takagi, Y., Nakajima, O. and Kobayashi, M.
  • Journal Title: Genes Cells Volume: 20 Issue: 7 Pages: 590-600
  • DOI: 10.1111/gtc.12249
  • NAID: 120007135764
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] PRMT8 as a phospholipase regulates Purkinje cell dendritic arborization and motor coordination. (2015)
  • Author(s): Kim, J.D., Park, K.E., Ishida, J., Kako, K., Hamada, J., Kani, S., Takeuchi, M., Namiki, K., Fukui, H., Fukuhara, S., Hibi, M,. Kobayashi, M., Kanaho, Y., Kasuya, Y., Mochizuki, N., Fukamizu, A.
  • Journal Title: Sci. Adv. Volume: 1 Issue: 11
  • DOI: 10.1126/sciadv.1500615
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Lateralization, maturation, and anteroposterior topography in the lateral habenula revealed by ZIF268/EGR1 immunoreactivity and labeling history of neuronal activity. (2015)
  • Author(s): Ichijo H, Hamada M, Takahashi S, Kobayashi M, Nagai T, Toyama T, Kawaguchi M.
  • Journal Title: Neurosci Res Volume: 95 Pages: 27-37
  • DOI: 10.1016/j.neures.2015.01.005
  • NAID: 120005539542
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Heme-mediated inhibition of Bach1 regulates the liver specificity and transience of the Nrf2-dependent induction of zebrafish heme oxygenase 1. (2015)
  • Author(s): Fuse, Y., Nakajima, H., Nakajima-Takagi, Y., Nakajima, O. and Kobayashi, M.
  • Journal Title: Genes Cells Volume: 未定
  • NAID: 120007135764
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Sulforaphane-induced Nrf2 activation supresses genetic endoplasmic reticulum stress in zebrafish. (2018)
  • Author(s): Nguyen, V.T., Mukaigasa, K. and Kobayashi, M.
  • Organizer: 第41回日本分子生物学会年会
• [Presentation] 種々スパイス由来ファイトケミカルがもつ酸化ストレス及び亜ヒ酸毒性に対する予防活性の違い (2018)
  • Author(s): 遠藤優佳、村木恭二、小林麻己人
  • Organizer: 第4回ゼブラフィッシュ・メダカ創薬研究会
• [Presentation] The role of histone demethylase LSD1 in zebrafish definitive hematopoiesis. (2018)
  • Author(s): Tamaoki, J., Kobayashi, I. and Kobayashi, M.
  • Organizer: 第80回日本血液学会学術集会,
• [Presentation] Evaluation of anti-oxidative and anti-arsenic potential of phytochemicals derived from spice plants using zebrafish. (2018)
  • Author(s): Endo, Y., Muraki, K. and Kobayashi, M.
  • Organizer: TGSW2018
  • Int'l Joint Research
• [Presentation] Primitive hematopoiesis does not require LSD1 demethylation activity in zebrafish. (2018)
  • Author(s): Tamaoki, J., Takeuchi, M. and Kobayashi, M.
  • Organizer: TGSW2018
  • Int'l Joint Research
• [Presentation] he different stress-sensing activities of Keap1a and Keap1b in zebrafish. (2018)
  • Author(s): Nguyen, V.T., Otsubo, S., Fuse, Y. and Kobayashi, M.
  • Organizer: TGSW2018
• [Presentation] Demethylase-independent function of LSD1 in zebrafish primitive hematopoiesis. (2018)
  • Author(s): Tamaoki, J., Takeuchi, M. and Kobayashi, M.
  • Organizer: 第24回小型魚類研究会
• [Presentation] he protective role of Nrf2 in response to phosphomannomutase 2 mutation-induced endoplasmic reticulum stress in zebrafish. (2018)
  • Author(s): Nguyen, V.T., Mukaigasa, K. and Kobayashi, M.
  • Organizer: The 13th International Zebrafish Conference
  • Int'l Joint Research
• [Presentation] The role of histone demethylase LSD1 in the development of hematopoietic stem cells in zebrafish. (2018)
  • Author(s): Tamaoki, J., Kobayashi, I. and Kobayashi, M.
  • Organizer: 第70回日本細胞生物学会 第51回日本発生生物学会合同大会
• [Presentation] LSD1-mediated regulation of Gata genes in primitive and definitive hematopoiesis. (2018)
  • Author(s): Tamaoki, J., Takeuchi, M. and Kobayashi, M.
  • Organizer: IUBMB Focused Meeting on GATA Transcription Factors
  • Int'l Joint Research
• [Presentation] Nrf2-dependent anti-oxidative action of an anti-arthritic drug, auranofin: the possible repositioning for oxidative stress-related diseases. (2018)
  • Author(s): Fuse,Y., Nguyen, V.T., Endo, Y. and Kobayashi, M.
  • Organizer: The Society of Toxicology 58th Anual meeting and ToxExpo
  • Int'l Joint Research
• [Presentation] ゼブラフィッシュを活用したNrf2システムの研究と応用 (2017)
  • Author(s): 小林麻己人
  • Organizer: 第90回日本薬理学会年会
  • Place of Presentation: 長崎新聞文化ホール（長崎県長崎市）
  • Year and Date: 2017-03-15
  • Invited
• [Presentation] ゼブラフィッシュを用いたNrf2システムの解析：医療応用に向けた新たな課題との遭遇 (2017)
  • Author(s): 布施雄士、Vu Thanh Nguyen、玉置隼也、小林麻己人
  • Organizer: 転写研究会 冬の若手ワークショップ2017
  • Place of Presentation: 一宮シーサイドオーツカ（千葉県一宮町）
  • Year and Date: 2017-01-30
• [Presentation] ゼブラフィッシュモデルの活用により発見した毒性の低いNrf2活性化剤による亜ヒ酸毒性軽減効果 (2017)
  • Author(s): 布施雄士、遠藤優佳、Vu Thanh Nguyen、小林麻己人
  • Organizer: 第23回ヒ素シンポジウム
• [Presentation] Study of the Keap1-Nrf2 pathway using a zebrafish model. (2017)
  • Author(s): Makoto Kobayashi
  • Organizer: International Meeting on Non-Mammalian Models in Biomedical Research: Current Status and Future
  • Int'l Joint Research / Invited
• [Presentation] Identification of new Nrf2-target genes in zebrafish embryos. (2017)
  • Author(s): Nguyen, V.T., Mukaigasa, K., Fuse, Y. and Kobayashi, M.
  • Organizer: TGSW2017
  • Int'l Joint Research
• [Presentation] The ConservedNrf2-mediated gene regulation of proteasome subunits and glucose metabolism in zebrafish. (2017)
  • Author(s): Nguyen, V.T., Fuse, Y., Tamaoki, J. and Kobayashi, M.
  • Organizer: The 10th Vietnam-Japan Scientific Exchange Meeting
  • Int'l Joint Research
• [Presentation] Exploration of novel gene- and tissue-specific induction systems in response to Nrf2-activating compounds. (2017)
  • Author(s): Fuse, Y. and Kobayashi, M.
  • Organizer: 第23回小型魚類研究会
• [Presentation] チオレドキシン過剰発現による重金属毒性の軽減:トランスジェニックゼブラフィッシュを用いたin vivo解析 (2017)
  • Author(s): 布施雄士、Vu Thanh Nguyen、小林麻己人
  • Organizer: 第44回日本毒性学会学術年会
• [Presentation] Nrf2システムの基礎 (2017)
  • Author(s): 小林麻己人
  • Organizer: 第70回日本酸化ストレス学会学術集会
  • Invited
• [Presentation] 酸化ストレス障害に対するゼブラフィッシュNrf2活性化による保護作用 (2017)
  • Author(s): 小林麻己人、Nguyen Vu Thanh、布施雄士
  • Organizer: 第70回日本酸化ストレス学会学術集会
• [Presentation] 多様な薬剤によって変化するNrf2標的遺伝子の組織特異的誘導プロファイル (2017)
  • Author(s): 布施雄士、小林麻己人
  • Organizer: 平成29年度日本生化学会関東支部例会
• [Presentation] Sulforaphane reduces genetic endoplasmic reticulum stress in zebrafish through the Nrf2 pathway. (2016)
  • Author(s): Nguyen, V.T., Mukaigasa, K., Fuse, Y. and Kobayashi, M.
  • Organizer: The 19th Asia-Pacific International Molecular Biology Network (A-IMBN) Annual Conference
  • Place of Presentation: Ho Chi Minh City, Vietnam
  • Year and Date: 2016-10-30
  • Int'l Joint Research
• [Presentation] Activation of Nrf2 attenuates genetic endoplasmic reticulum stress in zebrafish (2016)
  • Author(s): Nguyen, V.T., Mukaigasa, K., Fuse, Y. and Kobayashi, M.
  • Organizer: 第89回日本生化学会大会
  • Place of Presentation: 仙台国際センター（宮城県仙台市）
  • Year and Date: 2016-09-25
• [Presentation] 亜ヒ酸の毒性に対するNrf2依存的生体防御の遺伝学的解析 (2016)
  • Author(s): 布施雄士、Vu Thanh Nguyen、小林麻己人
  • Organizer: 第89回日本生化学会大会
  • Place of Presentation: 仙台国際センター（宮城県仙台市）
  • Year and Date: 2016-09-25
• [Presentation] Nrf2-dependent attenuation of genetic endoplasmic reticulum stress in zebrafish (2016)
  • Author(s): Nguyen, V.T., Mukaigasa, K., Fuse, Y. and Kobayashi, M.
  • Organizer: TGSW2016
  • Place of Presentation: つくば国際会議場（茨城県つくば市）
  • Year and Date: 2016-09-17
  • Int'l Joint Research
• [Presentation] Genetic evidence of Nrf2-dependent protection against heavy metal toxicity (2016)
  • Author(s): Fuse, Y., Nguyen, V.T. and Kobayashi, M.
  • Organizer: TGSW2016
  • Place of Presentation: つくば国際会議場（茨城県つくば市）
  • Year and Date: 2016-09-17
  • Int'l Joint Research
• [Presentation] Quality of visual chart designs created by Japanese researchers and engineers (2016)
  • Author(s): Tanaka, S., Kobayashi, M. and Miwa, Y.
  • Organizer: TGSW2016
  • Place of Presentation: つくば国際会議場（茨城県つくば市）
  • Year and Date: 2016-09-17
  • Int'l Joint Research
• [Presentation] Nrf2-dependent protection against ROS-generating heavy metals. (2016)
  • Author(s): Fuse, Y., Nguyen, V.T. and Kobayashi, M.
  • Organizer: 第22回小型魚類研究会
  • Place of Presentation: 岡崎コンファレンスセンター（愛知県岡崎市）
  • Year and Date: 2016-08-20
• [Presentation] Analysis of the lsd1;etv2 compound mutant reveals roles and regulatory mechanisms of LSD1 in zebrafish hematopoiesis (2016)
  • Author(s): Tamaoki, J., Takeuchi, Mik., Takeuchi, Mih. and Kobayashi, M.
  • Organizer: 第22回小型魚類研究会
  • Place of Presentation: 岡崎コンファレンスセンター（愛知県岡崎市）
  • Year and Date: 2016-08-20
• [Presentation] LSD1-dependent shutdown of etv2 promotes hematopoietic differentiation in hemangioblasts (2016)
  • Author(s): Kobayashi, M., Tamaoki, J., Fuse, Y. and Takeuchi, M.
  • Organizer: The Allied Genetics 2016 Conference
  • Place of Presentation: Orland, USA
  • Year and Date: 2016-07-13
  • Int'l Joint Research
• [Presentation] Involvement of the p62-Nrf2 pathway as a protection mechanism against Spns1 deficiency in zebrafish (2016)
  • Author(s): Khan, A., Shanshan, L., Sasaki, T., Kobayashi, M. and Kishi, S.
  • Organizer: The Allied Genetics 2016 Conference
  • Place of Presentation: Orland, USA
  • Year and Date: 2016-07-13
  • Int'l Joint Research
• [Presentation] ゼブラフィッシュを用いた亜ヒ酸の急性毒性に対するNrf2依存的防御機構の遺伝学的機能解析 (2016)
  • Author(s): 布施雄士、Vu Thanh Nguyen、小林麻己人
  • Organizer: 第43回日本毒性学会学術年会
  • Place of Presentation: ウインクあいち（愛知県名古屋市）
  • Year and Date: 2016-06-29
• [Presentation] Pmm2変異で自然発症した小胞体ストレスのNrf2システムによる緩和 (2016)
  • Author(s): 小林麻己人、Vu Thanh Nguyen、布施雄士、向笠勝貴
  • Organizer: 平成28年度日本生化学会関東支部例会
  • Place of Presentation: 自治医科大学（栃木県下野市）
  • Year and Date: 2016-06-11
• [Presentation] 亜ヒ酸の急性毒性に対するNrf2システム依存的な生体防御 (2016)
  • Author(s): 布施雄士、Vu Thanh Nguyen、小林麻己人
  • Organizer: 平成28年度日本生化学会関東支部例会
  • Place of Presentation: 自治医科大学（栃木県下野市）
  • Year and Date: 2016-06-11
• [Presentation] Nrf2-dependent Protection against Acute Arsenite Toxicity in Zebrafish. (2016)
  • Author(s): Fuse, Y., Nguyen, V.T. and Kobayashi, M.
  • Organizer: International Meeting on Aquatic Model Organisms for Human Disease and Toxicology Research
  • Place of Presentation: 岡崎、岡崎カンファレンスセンター
  • Year and Date: 2016-03-18
• [Presentation] A Zebrafish Model of Human Congenital Disorder of Glycosylation Ia. (2016)
  • Author(s): Mukaigasa,M., Nguyen, V.T., Sato, H., Fuse, Y. and Kobayashi, M.
  • Organizer: International Meeting on Aquatic Model Organisms for Human Disease and Toxicology Research
  • Place of Presentation: 岡崎、岡崎カンファレンスセンター
  • Year and Date: 2016-03-18
• [Presentation] 高濃度亜ヒ酸暴露時における生体防御機構Nrf2システムの遺伝学的機能解析 (2016)
  • Author(s): 布施雄士、Vu Thanh Nguyen、小林麻己人
  • Organizer: 転写研究会 冬の若手ワークショップ2016
  • Place of Presentation: 山中湖、山中湖畔 ホテル清渓
  • Year and Date: 2016-02-04
• [Presentation] ゼブラフィッシュNrf2システムを活用した毒性研究 (2015)
  • Author(s): 小林麻己人、大坪士朗、布施雄士
  • Organizer: 第１回ゼブラフィッシュ創薬研究会
  • Place of Presentation: 津、三重大学 環境・情報科学館
  • Year and Date: 2015-11-06
• [Presentation] The heme-Bach1 axis determines the tissue specificity of the Nrf2-dependent heme oxygenase 1 induction. (2015)
  • Author(s): Fuse, Y., Nakajima, H., Nakajima-Takagi, Y. and Kobayashi, M.
  • Organizer: TGSW2015
  • Place of Presentation: つくば、筑波国際会議場
  • Year and Date: 2015-09-28
  • Int'l Joint Research
• [Presentation] Histone demethylase LSD1 is important for the endodermal organ development in zebrafish. (2015)
  • Author(s): Andrea, C.S., Fuse, Y. and Kobayashi, M.
  • Organizer: TGSW2015
  • Place of Presentation: つくば、筑波国際会議場
  • Year and Date: 2015-09-28
  • Int'l Joint Research
• [Presentation] Dual regulation of heme oxygenase 1 transcription by the Nrf2 and heme-Bach1 axis. (2015)
  • Author(s): Fuse,Y., Nakajima, H., Nakajima-Takagi, Y. and Kobayashi, M.
  • Organizer: 第21回小型魚類研究会
  • Place of Presentation: 大阪、大阪大学銀杏会館
  • Year and Date: 2015-09-19
• [Presentation] Keap1-Nrf2システムの化学物質およびストレス応答機序 (2015)
  • Author(s): 小林麻己人
  • Organizer: 第42回日本毒性学会学術年会
  • Place of Presentation: 金沢、石川県立音楽堂 他
  • Year and Date: 2015-06-29
  • Invited
• [Presentation] ゼブラフィッシュを用いた環境ストレス応答の研究 (2015)
  • Author(s): 小林麻己人
  • Organizer: 第42回日本毒性学会学術年会
  • Place of Presentation: 金沢、石川県立音楽堂 他
  • Year and Date: 2015-06-29
  • Invited
• [Presentation] Histone demethylase LSD1 is required for normal development of endodermal organs in zebrafish. (2015)
  • Author(s): Andrea, C.S., Fuse, Y. and Kobayashi, M.
  • Organizer: 第46回日本発生生物学会年会
  • Place of Presentation: つくば、筑波国際会議場
  • Year and Date: 2015-06-02
• [Presentation] Nrf2活性化時における転写抑制因子Bach1の機能解析および2種類の魚類Bach1ホモログの比較解析． (2015)
  • Author(s): 布施雄士、中島瞳、中島やえ子、小林麻己人．
  • Organizer: 転写研究会 冬の若手ワークショップ2015
  • Place of Presentation: 伊香保
  • Year and Date: 2015-02-05 – 2015-02-07
• [Presentation] Bach1 and heme are involved in liver-specific and transient induction of heme oxygenase 1 in zebrafish. (2014)
  • Author(s): Fuse, Y., Nakajima, H., Nakajima-Takagi, Y. and Kobayashi, M.
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] Liver-specific induction of heme oxigenase 1 is mediated by heme and Bach1 in zebrafish. (2014)
  • Author(s): Fuse, Y., Nakajima, H., Nakajima-Takagi, Y. and Kobayashi, M.
  • Organizer: 第20回小型魚類研究会
  • Place of Presentation: 東京
  • Year and Date: 2014-09-20 – 2014-09-21
• [Remarks]
  • URL: http://www.md.tsukuba.ac.jp/MDBiology/mdbiol.index.html
• [Remarks] 筑波大学医学医療系・分子発生生物学研究室
  • URL: http://www.md.tsukuba.ac.jp/MDBiology/mdbiol.index.html
• [Remarks] 筑波大学医学医療系 分子発生生物学研究室
  • URL: http://www.md.tsukuba.ac.jp/MDBiology/mdbiol.index.htm