| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Three approaches were utilized to examine the feasibility of biotechnological application of CaMKII memory function: biochemical experiments, cellar and molecular biological experiments, and computer simulation. I developed the insect cell expression system of CaMKII-FRET, and also developed the optical observation/simulation system of target molecules in HeLa cells. The CaMKII memory system is also applicable. Also, I computationally simulated the reaction-diffusion system of the memory-related molecules in morphologically realistic dendrites and spines, to examine localization of those molecules.
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