Biotechnological application of memory-related molecules in the brain
Project/Area Number |
26540154
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Life / Health / Medical informatics
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Research Institution | Kyoto University |
Principal Investigator |
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Project Period (FY) |
2014-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
Keywords | 生体分子メモリ / シミュレーション / 再構成系 / CaMKII |
Outline of Final Research Achievements |
Three approaches were utilized to examine the feasibility of biotechnological application of CaMKII memory function: biochemical experiments, cellar and molecular biological experiments, and computer simulation. I developed the insect cell expression system of CaMKII-FRET, and also developed the optical observation/simulation system of target molecules in HeLa cells. The CaMKII memory system is also applicable. Also, I computationally simulated the reaction-diffusion system of the memory-related molecules in morphologically realistic dendrites and spines, to examine localization of those molecules.
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Report
(5 results)
Research Products
(8 results)
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[Journal Article] A critical time window for dopamine actions on the structural plasticity of dendritic spines.2014
Author(s)
Yagishita, S., Hayashi-Takagi, A., Ellis-Davies, G.C.R., Urakubo, H., Ishii, S., & Kasai, H.
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Journal Title
Science
Volume: 345
Issue: 6204
Pages: 1616-1620
DOI
Related Report
Peer Reviewed
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[Journal Article] A statistical method of identifying interactions in neuron-glia systems based on functional multicell Ca2+ imaging.2014
Author(s)
Nakae, K., Ikegaya, Y., Ishikawa, T., Oba, S., Urakubo, H., Koyama, M. & Ishii, S.
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Journal Title
PLoS Computational Biology
Volume: 10
Issue: 11
Pages: e1003949-e1003949
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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