Impact and response to DNA damage in mitosis

Research Project

Project/Area Number	26550027
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Risk sciences of radiation and chemicals
Research Institution	Kyoto University
Principal Investigator	Matsumoto Tomohiro 京都大学, 放射線生物研究センター, 教授 (80212223)
Project Period (FY)	2014-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000) Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000) Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords	有糸分裂期 / テロメア / 染色体凝縮 / 酵母 / 放射線感受性 / クロマチン / 損傷修復 / スピンドルチェックポイント
Outline of Final Research Achievements	Mitosis is a cell cycle stage in which the radiation sensitivity is higher than any other stages, but the underlying mechanisms are still unclear. In this research project, we used the fission yeast model system and found the followings: 1) Some of the stress response genes, including DNA repair gene around telomere are normally silenced. 2) This silencing requires a chromosome-condensing factor and a kinase that phosphorylates the condensing factor in mitosis. We therefore found a novel mechanism of gene silencing that likely accounts for a low activity of DNA repair in mitosis.