Research Project
Grant-in-Aid for Challenging Exploratory Research
It is known that histone modifications including the phosphorylatin, acetylation, ubiquitination and methylation have been detected after induction of DNA damage. However, it is unclear whether these modifications are removed or still observed after DNA damage-repair. Thus we investigated the status of these modifications including the phosphorylated H2AX at the DNA damage-repaired sites by the chromatin immunoprecipitation (Chromation IP). As a result, the phosphorylated histone H2AX was decreased as DNA damage is repaired. We are still analyzing the status of some other histone modifications by using chromatin IP method. As for the chromatin status at the DNA damage site by I-Sce1 by using real-time PCR for the sensitivity for MNase, we observed the chromatin opening at the damage site. We will analyze the chromatin status after DNA repair in the near future.
All 2016 2015 2014
All Journal Article (7 results) (of which Peer Reviewed: 7 results, Open Access: 4 results, Acknowledgement Compliant: 2 results) Presentation (12 results)
Mol. Cell. Biol.
Volume: Epub ahead of print Issue: 10 Pages: 1595-1607
10.1128/mcb.01085-15
Toxicol. Sci.
Volume: 150 Issue: 2 Pages: 1-12
10.1093/toxsci/kfw039
Mol. cell. Biol.
Volume: 35 Issue: 24 Pages: 4147-4157
10.1128/mcb.00757-15
Nucleic Acids Res
Volume: 44(2) Issue: 2 Pages: 636-647
10.1093/nar/gkv967
120005818255
Genes Cells.
Volume: 20 Issue: 9 Pages: 681-94
10.1111/gtc.12262
Radiat. Environ. Biophys.
Volume: 54 Issue: 4 Pages: 403-411
10.1007/s00411-015-0608-3
Scientific Rep.
Volume: 4 Issue: 1 Pages: 4863-4863
10.1038/srep04863
