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Development of a reliable analytical method for endogenous hormones applicable to various animal species

Research Project

Project/Area Number 26550048
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Environmental impact assessment
Research InstitutionEhime University

Principal Investigator

Kunisue Tatsuya  愛媛大学, 沿岸環境科学研究センター, 教授 (90380287)

Co-Investigator(Renkei-kenkyūsha) TANABE SHINSUKE  愛媛大学, 沿岸環境科学研究センター, 教授 (60116952)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords甲状腺ホルモン / 機器分析 / 遊離型 / タンパク結合型 / ステロイドホルモン
Outline of Final Research Achievements

The present study aimed to develop a sensitive and reliable analytical method for endogenous hormones, especially free thyroid hormones (THs) that play important role in the brain development, in serum/plasma of various animal species using ultrafiltration (UF) in combination with ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), which can resolve the interferences encountered due to the nonspecific reactivity of anti-hormone antibodies used in immunoassay (IA) methods. UF at 1100 × g and 37°C for 30 minutes with a 30 kDa ultrafiltration device (Centrifree YM-30) yielded excellent precision. Acceptable accuracy (recovery: 100-110%) and intra- and inter-day precision (CV: <10% and <12%, respectively) were obtained, when triplicate analyses in three different days were conducted using bovine serum. The developed analytical method was successfully applied to the determination of free THs in plasma samples of humans and cats.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (9 results)

All 2016 2015 2014

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (7 results) (of which Int'l Joint Research: 4 results)

  • [Journal Article] Organohalogens and their hydroxylated metabolites in the blood of pigs from an open waste dumping site in south India: Association with hepatic cytochrome P4502015

    • Author(s)
      Mizukawa H, Nomiyama K, Kunisue T, Watanabe MX, Subramanian A, Iwata H, Ishizuka M, Tanabe S
    • Journal Title

      Environ Res

      Volume: 138 Pages: 255-263

    • DOI

      10.1016/j.envres.2015.02.012

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Occurrence of Perchlorate and Thiocyanate in Human Serum From E-Waste Recycling and Reference Sites in Vietnam : Association With Thyroid Hormone and Iodide Levels2014

    • Author(s)
      Akifumi Eguchi, Tatsuya Kunisue, Qian Wu, Pham Thi Kim Trang, Pham Hung Viet, Kurunthachalam Kannan, Shinsuke Tanabe
    • Journal Title

      Archives of environmental contamination and toxicology

      Volume: (in press) Issue: 1 Pages: 29-41

    • DOI

      10.1007/s00244-014-0021-y

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] A sensitive and reliable analytical method for free thyroid hormones in animal serum using ultrafiltration in combination with UHPLC-MS/MS2016

    • Author(s)
      Tanoue, R., Kume, I., Yamamoto, Y., Nomiyama, K., Tanabe, S., Kunisue, T.
    • Organizer
      SETAC North America 37th Annual Meeting/7th SETAC World Congress
    • Place of Presentation
      Orlando, USA
    • Year and Date
      2016-11-06
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Accumulation features of organohalogen compounds and their hydroxylated metabolites in pet cats and dogs: effects on thyroid hormones homeostasis2016

    • Author(s)
      Nomiyama, K., Yamamoto, Y., Mizukawa, H., Eguchi, A., Nakayama, S. M., Ikenaka, Y., Ishizuka, M., Kunisue, T., Tanabe, S.
    • Organizer
      SETAC North America 37th Annual Meeting/7th SETAC World Congress
    • Place of Presentation
      Orlando, USA
    • Year and Date
      2016-11-06
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Contamination status of organohalogen compounds and their hydroxylated metabolites in pet cats and dogs: effects on thyroid hormones homeostasis2016

    • Author(s)
      Nomiyama, K., Yamamoto, Y., Mizukawa, H., Takaguchi, K., Takiguchi, M., Eguchi, A., Nakayama, S., Ikenaka, Y., Ishizuka, M., Kunisue, T., Tanabe, S.
    • Organizer
      36th International Symposium on Halogenated Persistent Organic Pollutants
    • Place of Presentation
      Firenze, Italy
    • Year and Date
      2016-08-28
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 有機ハロゲン化合物によるコンパニオンアニマルの汚染実態と甲状腺ホルモンへの影響評価2016

    • Author(s)
      野見山 桂・山本恭央・水川葉月・江口哲史・滝口満喜・中山翔太・池中良徳・石塚 真由美・国末 達也・田辺信介
    • Organizer
      第25回環境化学討論会
    • Place of Presentation
      新潟コンベンションセンター(新潟県・新潟市)
    • Year and Date
      2016-06-08
    • Related Report
      2016 Annual Research Report
  • [Presentation] Contamination status of organohalogen compounds and their hydroxylated metabolites in pet cats: Effects on thyroid hormones homeostasis2015

    • Author(s)
      Yamamoto, Y., Nomiyama, K., Mizukawa, H., Takiguchi, M., Ishizuka, M., Kunisue, T. Tanabe, S.
    • Organizer
      Society of Environmental Toxicology and Chemistry (SETAC) North America 36th Annual Meeting
    • Place of Presentation
      Salt Lake City, Utah, USA
    • Year and Date
      2015-11-01
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 遊離型甲状腺ホルモンの高精度機器分析法の開発2015

    • Author(s)
      久米伊万里・田上瑠美・田辺信介・国末達也
    • Organizer
      第24回環境化学討論会
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Year and Date
      2015-06-24
    • Related Report
      2015 Research-status Report
  • [Presentation] ペットネコにおける有機ハロゲン化合物および水酸化代謝物の汚染実態と甲状腺ホルモン恒常性への影響2015

    • Author(s)
      山本恭央・野見山 桂・水川葉月・滝口満喜・石塚真由美・国末達也・田辺信介
    • Organizer
      第24回環境化学討論会
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Year and Date
      2015-06-24
    • Related Report
      2015 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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