| Project/Area Number |
26550081
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental risk control and evaluation
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KATAYAMA Hiroyuki 東京大学, 工学(系)研究科(研究院), 准教授 (00302779)
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| Co-Investigator(Renkei-kenkyūsha) |
Kurisu Futoshi 東京大学, 大学院工学系研究科, 准教授 (30312979)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | ウイルス / PCR / 不活化 / 誤陽性 / 消毒 / 細菌 / RNA分解 |
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| Outline of Final Research Achievements |
PCR based detection cannot distinguish between infectious and non-infectious viruses. This research aimed to develop such a method to selectively remain infectious virion prior to PCR based detection. Various bacterial strains were applied to decompose naked viral RNA. However, no bacteria achieved a good performance to degrade RNA in a short time, determined by RT-PCR. Then,instead of bacteria, platinum reagents were tested for the same purpose. As a result, cis-DDP, one of platinum reagents, worked to remove RNA but remain intact virus RNA.
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