| Project/Area Number |
26550105
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Design and evaluation of sustainable and environmental conscious system
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| Research Institution | Kumamoto Industrial Research Institute |
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Principal Investigator |
Tanaka Ryoichi 熊本県産業技術センター(ものづくり室、材料・地域資源室、食品加工室), その他部局等, 研究主任 (50468063)
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| Co-Investigator(Renkei-kenkyūsha) |
HORI Tomoyuki 産業技術総合研究所, 環境管理技術研究部門, 主任研究員 (20509533)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | Stable isotope probing / 16S rRNA gene sequencing / 長鎖脂肪酸分解 / Activated sludge / Membrane bioreactor / Stable Isotope Probing / LCFA degradation / RNA-SIP / 16S deep sequencing |
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| Outline of Final Research Achievements |
We focused on the long chain fatty acid degradation that is one of rate-limiting step on lipid degrading process in the activated sludge treatment, unknown microorganism involved in degrading palmitic acid in the activated sludge was identified with a new fusion method of RNA-stable isotope probing (SIP) and 16S rRNA gene deep sequencing. Furthermore, rare species of microorganism taking in palmitic acid were revealed. The new powerful method of RNA-SIP and next generation sequencer technology was demonstrated to be useful in ultra-high sensitive identification of unknown microorganisms in environment.
To develop control technology of a high performance membrane bioreactor (MBR) for lipid-degradation, a small scale MBR was operated using wastewater including the olive oil, and microbes induced fouling and functional decline on the MBR in oily wastewater treatment were revealed.
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