| Project/Area Number |
26560047
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Eating habits
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| Research Institution | Nagoya University of Arts and Sciences |
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Principal Investigator |
MASAKI Takeshi 名古屋学芸大学, 管理栄養学部, 講師 (10387912)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | アレルギー・ぜんそく / 蛋白質 / 酵素 / 植物 / 食品 / ダイズ / 発芽 / プロテアーゼ |
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| Outline of Final Research Achievements |
In this research, major allergic protein, Gly m Bd 30K contained in soybean etiolated seedlings started to decrease at 14 days after imbibition although most of seed storage protein disappeared until 5 days. It shows that Gly m Bd 30K plays a different role from other seed protein stored as source of nitrogen, carbon, energy. In addition, it is likely that Gly m Bd 30K is degraded by another protease from that degrade seed storage protein. And we revealed that Gly m Bd 30K in solution prepared from soybean 15 days seedlings decreased when it was incubated under 30℃. Such in vitro decrease of Gly m Bd 30K was disrupted by aspartic protease inhibitor but not antiseptic agent and proteasome inhibitor. It indicates that some kind of aspartic protease is activated for degradation of Gly m Bd 30K.
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