Development of a simple device named "short-strip cellulose paper electrophoresis" for food safety
Project/Area Number |
26560069
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Eating habits
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Research Institution | Aichi Gakusen University |
Principal Investigator |
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Keywords | ヒスタミン / ヒスタミン食中毒 / ろ紙電気泳動 / ペーパー分析デバイス / スマートフォン / ポリアミン / ヒスチジン |
Outline of Final Research Achievements |
A method named short-strip cellulose paper electrophoresis was developed for the determination of histamine (HA), and for the screening of histaminases or diamine oxidase (DAO) inhibitor in food in order to prevent HA food poisoning. Using this method after optimizing it, we were able to analyze HA in food samples like 5 brands of soy sauce, 3 brands of first-fish and 5 kinds of raw-fish. We demonstrated the inhibition of DAO with ethanol. In addition, we also developed application software running on a smart-phone (Android 4.3) to read out the amount of HA in digital format.
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Report
(4 results)
Research Products
(13 results)