Identification of diet contents regulating RNA methylation and those effects on fetal growth

• Project/Area Number: 26560076
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Eating habits
• Research Institution: National Center for Child Health and Development
• Principal Investigator: Kawai Tomoko 国立研究開発法人国立成育医療研究センター, 周産期病態研究部, 室長 (40423404)
• Research Collaborator: Taniguchi Kosuke
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 胎児発育 / 胎盤 / RNAメチル化 / 出生体重 / 子宮内栄養環境 / エピトランスクリプトーム / ヒト胎盤

Outline of Final Research Achievements

Adenine methyl modification of mRNA is known to regulate mRNA fates and alter the mRNA function. The reports elucidating mRNA methylation roles in the specified cells are increasing in an exponential manner. On the other hands, the studies exploring the mRNA methylation status at transcriptome-wide manner in vivo are limited. We investigated human placental transcriptome-wide methylation status and revealed that placental post-transcriptional gene expression regulation mechanism through mRNA methylation might be associated with fetal growth.

Research Products

• [Journal Article] Increased epigenetic alterations at the promoters of transcriptional regulators following inadequate maternal gestational weight gain. (2015)
  • Author(s): Kawai T, Yamada T, Abe K, Okamura K, Kamura H, Akaishi R, Minakami H, Nakabayashi K, Hata K.
  • Journal Title: Scientific reports Volume: 5 Issue: 1 Pages: 14224-14224
  • DOI: 10.1038/srep14224
  • Peer Reviewed / Open Access
• [Journal Article] Gene expression profiling of white adipose tissue reveals paternal transmission of proneness to obesity. (2015)
  • Author(s): Morita S, Nakabayashi K, Kawai T, Hayashi K, Horii T, Kimura M, Kamei Y, Ogawa Y, Hata K, Hatada I
  • Journal Title: Sci. Rep. Volume: 6 Issue: 1 Pages: 21693-21693
  • DOI: 10.1038/srep21693
  • Peer Reviewed / Open Access
• [Journal Article] Lists of HumanMethylation450 BeadChip probes with nucleotide-variant information obtained from the Phase 3 data of the 1000 Genomes Project. (2015)
  • Author(s): Okamura K, Kawai T, Hata K, Nakabayashi K.
  • Journal Title: Genomics data Volume: 7 Pages: 67-9
  • DOI: 10.1016/j.gdata.2015.11.023
  • Peer Reviewed / Open Access
• [Journal Article] DNA methylation analysis of human myoblasts during in vitro myogenic differentiation: de novo methylation of promoters of muscle-related genes and its involvement in transcriptional down-regulation (2015)
  • Author(s): Miyata K, Miyata T, Nakabayashi K, Okamura K, Naito M, Kawai T, Takada S, Kato K, Miyamoto S, Hata K, Asahara H
  • Journal Title: Hum. Mol. Genet. Volume: 24 Issue: 2 Pages: 410-423
  • DOI: 10.1093/hmg/ddu457
  • Peer Reviewed / Open Access
• [Presentation] Epitranscriptome of human placental tissue. (2016)
  • Author(s): Taniguchi K, Kawai T, Okamura K, Ogata H, Ohashi M, Nakabayashi K, Hata K
  • Organizer: RNA 2016
  • Place of Presentation: 京都国際会館
  • Year and Date: 2016-06-30
  • Int'l Joint Research
• [Presentation] Placental epigenome vary in relation to inadequate gestational weight gain (2016)
  • Author(s): Tomoko Kawai, Takahiro Yamada, Kosei Abe, Kohji Okamura, Hiromi Kamura, Rina Akaishi, Hisanori Minakami, Kazuhiko Nakabayashi, Kenichiro Hata
  • Organizer: The 13th International Congress of Human Genetics
  • Place of Presentation: 京都 国立京都国際会館
  • Year and Date: 2016-04-05
  • Int'l Joint Research
• [Presentation] 妊娠中の体重増加量と胎盤エピゲノム変化 (2015)
  • Author(s): 河合智子、山田崇弘、安部晃生、岡村浩司、嘉村浩美、赤石理奈、水上尚典、中林一彦、秦健一郎
  • Organizer: 日本人類遺伝学会
  • Place of Presentation: 東京 京王プラザホテル
  • Year and Date: 2015-10-16