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Development of novel molecular imaging method for visualizing pluripotency of iPS cells

Research Project

Project/Area Number 26560200
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biomedical engineering/Biomaterial science and engineering
Research InstitutionUniversity of Tsukuba

Principal Investigator

KANO Hideaki  筑波大学, 数理物質系, 准教授 (70334240)

Co-Investigator(Kenkyū-buntansha) NISHMURA Ken  筑波大学, 医学医療系, 助教 (80500610)
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywordsラマン / CARS / iPS細胞 / 非染色
Outline of Final Research Achievements

Induced pluripotent stem cells (iPS cells) have potential to differentiate wide variety of tissues and organs. Recently, various kinds of studies have been carried out in order to apply iPS cells to tissue engineering and regenerative medicine. However, it is still difficult to find pluripotency of living iPS cells without staining or molecular tagging. In the present study, we have performed molecular vibrational imaging of iPS cells using coherent anti-Stokes Raman scattering (CARS) microscopy to explore the spectroscopic signatures of pluripotency. We used home-built multiplex coherent anti-Stokes Raman scattering (CARS) microspectroscopic system. As samples, we used different two kinds of iPS cell colony; 5- and 7-days ones after introducing four factor for the reprograming process. The intranuclear CARS spectral profiles in 5- and 7-days shows spectroscopic difference in two bands around 1560 and 790 cm-1. These might be useful to marker bands for pluripotency.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (10 results)

All 2016 2015 2014 Other

All Int'l Joint Research (1 results) Presentation (8 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results) Remarks (1 results)

  • [Int'l Joint Research] CNRS(France)

    • Related Report
      2015 Annual Research Report
  • [Presentation] iPS細胞の多能性評価に向けた非線形分光イメージング2016

    • Author(s)
      米山 弘亮、瀬川 尋貴、西村 健、福田 綾、久武 幸司、加納 英明
    • Organizer
      応用物理学会春季学術講演会
    • Place of Presentation
      東京工業大学(東京都目黒区)
    • Year and Date
      2016-03-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] 生体組織の非線形ラマン分光イメージング2015

    • Author(s)
      加納 英明、秋山 敏宏
    • Organizer
      医用光学・分光学系合同研究会、 Medical Optics& spectroscopy2015
    • Place of Presentation
      ホテルグランド市ヶ谷(東京都新宿区)
    • Year and Date
      2015-12-03
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 生細胞・生体組織を染めずに見る ~コヒーレント・ラマン分光による ラベルフリーイメージング~2015

    • Author(s)
      加納 英明,秋山 敏宏
    • Organizer
      日本レーザー医学総会
    • Place of Presentation
      栃木文化センター(栃木県宇都宮市)
    • Year and Date
      2015-10-24
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 非線形マルチモーダル顕微鏡を用いたiPS細胞のリプログラミング過程のin vivo追跡2015

    • Author(s)
      米山 弘亮,瀬川 尋貴,西村 健,福田 綾,久武 幸司,加納 英明
    • Organizer
      分子科学討論会
    • Place of Presentation
      東京工業大学(東京都目黒区)
    • Year and Date
      2015-09-16
    • Related Report
      2015 Annual Research Report
  • [Presentation] CARS Molecular Fingerprinting of iPS Cells-Toward Visualizing Pluripotency2015

    • Author(s)
      Hiroaki Yoneyama,Hiroki Segawa,Ken Nishimura,Aya Fukuda,Koji Hisatake,Hideaki Kano
    • Organizer
      ICAVS8 (8th International Conference on Advanced Vibrational spectroscopy
    • Place of Presentation
      ウィーン工科大学(ウィーン), オーストリア
    • Year and Date
      2015-07-12
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Nonlinear Multimodal spectral Imaging of Living Cells2015

    • Author(s)
      Hiroaki Yoneyama, Hiroki segawa, Ken Nishimura, Aya Fukuda, Koji Hisatake, and Hideaki Kano
    • Organizer
      The Third Taiwan International symposium on Raman Spectroscopy & TARS Summer School
    • Place of Presentation
      Sun Moon Lake(Nantou), Taiwan
    • Year and Date
      2015-06-30
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] CARS分光イメージング法によるiPS細胞の非染色・非標識イメージング2014

    • Author(s)
      米山弘亮、瀬川尋貴、西村健、福田綾、久武幸司、加納英明
    • Organizer
      分子科学討論会
    • Place of Presentation
      広島大学(東広島市)
    • Year and Date
      2014-09-24
    • Related Report
      2014 Research-status Report
  • [Presentation] iPS細胞の多能性可視化に向けたCARS分光イメージング法の開発2014

    • Author(s)
      米山弘亮、瀬川尋貴、西村健、福田綾、久武幸司、加納英明
    • Organizer
      分光学会年次講演会
    • Place of Presentation
      理化学研究所(和光市)
    • Year and Date
      2014-05-26
    • Related Report
      2014 Research-status Report
  • [Remarks] 加納研究室

    • URL

      http://www.bk.tsukuba.ac.jp/~CARS/

    • Related Report
      2015 Annual Research Report 2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2017-05-10  

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