Isolation strategy of iPS cells that enable to maintain under xeno feeder free condition.

• Project/Area Number: 26560219
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Tokyo Metropolitan University
• Principal Investigator: Taoka Masato 首都大学東京, 理工学研究科, 准教授 (60271160)
• Co-Investigator(Kenkyū-buntansha): IZUMI Tomonori 山口大学, 医学(系)研究科, 准教授 (00261694) ; SHIMAMOTO Akira 広島大学, 医歯薬保健学研究院, 准教授 (70432713)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: プロテオミクス / 糖鎖 / 細胞表面タンパク質 / 糖タンパク質 / 表面マーカー / iPS細胞

Outline of Final Research Achievements

Detailed knowledge of cell-surface proteins of iPS cells is necessary to isolate a well-defined population and enhance their translational potential. To identify and quantify proteins specific to membrane with high throughput, we developed a normal phase chromatographic approach with STAGE tip strategy. Through the approach, we identified over 1400 of cell-surface and N-linked glycoproteins from 500 micro g of proteins extracted from iPS cells cultured with xeno- and feeder-free condition. Compared them with the proteins on the surface of the original cells before obtaining pluripotency and embryoid body cells after differentiation by label-free proteomics quantitation, we obtained the 10 candidate proteins specific for iPS cells in xeno- and feeder-free condition.

Research Products

• [Journal Article] Global PROTOMAP Profiling to Search for Biomarkers of Early-Recurrent Hepatocellular Carcinoma (2014)
  • Author(s): Taoka, Masato; Morofuji, Noriaki; Yamauchi, Yoshio; Ojima, Hidenori; Kubota, Daisuke; Terukina, Goro; Nobe, Yuko; Nakayama, Hiroshi; Takahashi, Nobuhiro; Kosuge, Tomoo; Isobe, Toshiaki; Kondo, Tadashi
  • Journal Title: J. Proteome Res. Volume: 13 Issue: 11 Pages: 4847-4858
  • DOI: 10.1021/pr500262p
  • Peer Reviewed
• [Presentation] CELL-SURFACE PROTEOME：N型糖タンパク質を指標にした細胞表面タンパク質の簡便な同定法 (2015)
  • Author(s): 田岡万悟 藤田千春 山内芳雄 嶋本顕 礒辺俊明
  • Organizer: 日本プロテオーム学会2015年会
  • Place of Presentation: 熊本県熊本市
  • Year and Date: 2015-07-23