| Project/Area Number |
26560219
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Tokyo Metropolitan University |
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Principal Investigator |
Taoka Masato 首都大学東京, 理工学研究科, 准教授 (60271160)
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| Co-Investigator(Kenkyū-buntansha) |
IZUMI Tomonori 山口大学, 医学(系)研究科, 准教授 (00261694)
SHIMAMOTO Akira 広島大学, 医歯薬保健学研究院, 准教授 (70432713)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | プロテオミクス / 糖鎖 / 細胞表面タンパク質 / 糖タンパク質 / 表面マーカー / iPS細胞 |
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| Outline of Final Research Achievements |
Detailed knowledge of cell-surface proteins of iPS cells is necessary to isolate a well-defined population and enhance their translational potential. To identify and quantify proteins specific to membrane with high throughput, we developed a normal phase chromatographic approach with STAGE tip strategy. Through the approach, we identified over 1400 of cell-surface and N-linked glycoproteins from 500 micro g of proteins extracted from iPS cells cultured with xeno- and feeder-free condition. Compared them with the proteins on the surface of the original cells before obtaining pluripotency and embryoid body cells after differentiation by label-free proteomics quantitation, we obtained the 10 candidate proteins specific for iPS cells in xeno- and feeder-free condition.
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