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Isolation strategy of iPS cells that enable to maintain under xeno feeder free condition.

Research Project

Project/Area Number 26560219
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biomedical engineering/Biomaterial science and engineering
Research InstitutionTokyo Metropolitan University

Principal Investigator

Taoka Masato  首都大学東京, 理工学研究科, 准教授 (60271160)

Co-Investigator(Kenkyū-buntansha) IZUMI Tomonori  山口大学, 医学(系)研究科, 准教授 (00261694)
SHIMAMOTO Akira  広島大学, 医歯薬保健学研究院, 准教授 (70432713)
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsプロテオミクス / 糖鎖 / 細胞表面タンパク質 / 糖タンパク質 / 表面マーカー / iPS細胞
Outline of Final Research Achievements

Detailed knowledge of cell-surface proteins of iPS cells is necessary to isolate a well-defined population and enhance their translational potential. To identify and quantify proteins specific to membrane with high throughput, we developed a normal phase chromatographic approach with STAGE tip strategy. Through the approach, we identified over 1400 of cell-surface and N-linked glycoproteins from 500 micro g of proteins extracted from iPS cells cultured with xeno- and feeder-free condition. Compared them with the proteins on the surface of the original cells before obtaining pluripotency and embryoid body cells after differentiation by label-free proteomics quantitation, we obtained the 10 candidate proteins specific for iPS cells in xeno- and feeder-free condition.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (2 results)

All 2015 2014

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (1 results)

  • [Journal Article] Global PROTOMAP Profiling to Search for Biomarkers of Early-Recurrent Hepatocellular Carcinoma2014

    • Author(s)
      Taoka, Masato; Morofuji, Noriaki; Yamauchi, Yoshio; Ojima, Hidenori; Kubota, Daisuke; Terukina, Goro; Nobe, Yuko; Nakayama, Hiroshi; Takahashi, Nobuhiro; Kosuge, Tomoo; Isobe, Toshiaki; Kondo, Tadashi
    • Journal Title

      J. Proteome Res.

      Volume: 13 Issue: 11 Pages: 4847-4858

    • DOI

      10.1021/pr500262p

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] CELL-SURFACE PROTEOME:N型糖タンパク質を指標にした細胞表面タンパク質の簡便な同定法2015

    • Author(s)
      田岡万悟 藤田千春 山内芳雄 嶋本顕 礒辺俊明
    • Organizer
      日本プロテオーム学会2015年会
    • Place of Presentation
      熊本県熊本市
    • Year and Date
      2015-07-23
    • Related Report
      2015 Annual Research Report

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Published: 2014-04-04   Modified: 2017-05-10  

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