• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

in vito reconstruction of 3-dimensional brain tissue using human cells

Research Project

Project/Area Number 26560247
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biomedical engineering/Biomaterial science and engineering
Research InstitutionTohoku Institute of Technology

Principal Investigator

Suzuki Ikuro  東北工業大学, 大学院工学研究科, 准教授 (90516311)

Research Collaborator KIMURA Tsuyosi  
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
KeywordsヒトiPS細胞由来ニューロン / 3次元培養 / 脱細胞組織 / 神経細胞シート / 神経シート / 脱細胞脳 / 長期培養 / 薬効評価 / ヒト由来神経細胞
Outline of Final Research Achievements

This study aimed to develop the 3-dimensional culture techniques using decellualized brain tissue and layered neuronal sheet in order to construct a biological brain tissue model. We confirmed that human iPSC-derived neuronal networks were cultured on the prepared decellularized pig brain tissue. We also found that a solution of decellularized brain tissue promote the survival and the elongation of neurites in cultured human iPS cell-derived neurons. These results suggest that the culture method using decellularized tissue are useful to 3-dimensional brain tissue model. Furthermore, we found that hiPSC-derived neuron sheet with maintain function can be detached and layered using temperature sensitive substrate.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (13 results)

All 2017 2016 2015 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (9 results) (of which Int'l Joint Research: 5 results,  Invited: 1 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Physiological maturation and drug responses of human induced pluripotent stem cell-derived cortical neuronal networks in long-term culture.2016

    • Author(s)
      Odawara A, Katoh H, Matsuda N, Suzuki I
    • Journal Title

      Scientific Reports

      Volume: 6,26181 Issue: 1 Pages: 1-14

    • DOI

      10.1038/srep26181

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Induction of long-term potentiation and depression phenomena in human induced pluripotent stem cell-derived cortical neurons.2016

    • Author(s)
      Odawara A, Katoh H, Matsuda N, Suzuki I.
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 469(4) Issue: 4 Pages: 856-62

    • DOI

      10.1016/j.bbrc.2015.12.087

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 創薬・再生医療への展開を目指したヒトiPS細胞由来神経ネットワークの機能評価技術2017

    • Author(s)
      鈴木郁郎
    • Organizer
      第16回 日本再生医療学会総会
    • Place of Presentation
      宮城
    • Year and Date
      2017-03-07
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 脱細胞化スライス脳を用いたin vitro三次元脳組織の再構築2017

    • Author(s)
      木村剛, 猪狩優花, 中村奈緒子, 橋本良秀, 藤里俊哉, 小田原あおい, 鈴木郁郎, 岸田晶夫
    • Organizer
      第16回 日本再生医療学会総会
    • Place of Presentation
      宮城
    • Year and Date
      2017-03-07
    • Related Report
      2016 Annual Research Report
  • [Presentation] Reconstruction of brain circuit using decellularized brain tissue2016

    • Author(s)
      Ikuro Suzuki, Aoi Odawara, Yuuka Igari, Naoko Nakamura, Akio Kishida, Tsuyoshi Kimura
    • Organizer
      第1回生体医歯工学共同研究拠点国際シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2016-11-10
    • Related Report
      2016 Annual Research Report
  • [Presentation] In vitro 脳再構築を目指した脳ECMでの神経細胞培養2016

    • Author(s)
      木村剛, 猪狩優花, 中村奈緒子, 橋本良秀, 藤里俊哉, 鈴木郁郎, 岸田晶夫
    • Organizer
      第37回日本炎症・再生医学会
    • Place of Presentation
      京都
    • Year and Date
      2016-06-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] Induction of long-term potentiation and depression phenomena in hiPSC-derived cortical neuronal networks.2016

    • Author(s)
      A. Odawara, H. Katoh, N. Matsuda, I. Suzuki.
    • Organizer
      ISSCR International Symposium 2016
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      2016-03-22
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Electrophysiological maturation and drug responses in long-term culture of hiPSC-derived cortical neuronal networks.2016

    • Author(s)
      I. Suzuki. A. Odawara, H. Katoh, N. Matsuda
    • Organizer
      ISSCR International Symposium 2016
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      2016-03-22
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Pharmacological Responses in Cultured Human iPSC Derived Cortical Neurons Using Multi-Electrode Array.2016

    • Author(s)
      A. Odawara, H. Katoh, N. Matsuda, Y. Shi, Ryan Arant, H. Jiko, I. Suzuki.
    • Organizer
      Society of Toxicology
    • Place of Presentation
      New Orleans, USA
    • Year and Date
      2016-03-13
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Long-term electrophysiological activities and drug responses in cultured human iPSC derived neurons.2015

    • Author(s)
      A. Odawara, Y. Shi, H. Jiko, I. Suzuki.
    • Organizer
      Neuroscience 2015, Society for neuroscience
    • Place of Presentation
      Chicago, USA
    • Year and Date
      2015-10-17
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] In vitro electrophysiological drug testing using human induced pluripotent stem cell-derived neurons.2015

    • Author(s)
      I. Suzuki, A. Odawara, Y. Shi, H. Jiko.
    • Organizer
      ISSCR 2015, 13th Annual Meeting.
    • Place of Presentation
      Stockholm, Sweden
    • Year and Date
      2015-06-24
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Book] 特集:創薬とバイオマテリアル ~現状の組織・臓器別モデルと新しい三次元組織構築技術の応用~ 「ヒトiPS細胞由来ニューロンの長期培養と薬効評価技術」2015

    • Author(s)
      鈴木郁郎
    • Total Pages
      8
    • Publisher
      日本バイオマテリアル学会
    • Related Report
      2015 Research-status Report
  • [Remarks] ヒトiPS細胞由来大脳皮質ニューロンの長期活動計測と電気生理学的な薬剤応答の検出に成功

    • URL

      http://www.eis.tohtech.ac.jp/topics/20160519-01.html

    • Related Report
      2015 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi