in vito reconstruction of 3-dimensional brain tissue using human cells
| Project/Area Number |
26560247
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Tohoku Institute of Technology |
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Principal Investigator |
Suzuki Ikuro 東北工業大学, 大学院工学研究科, 准教授 (90516311)
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| Research Collaborator |
KIMURA Tsuyosi
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | ヒトiPS細胞由来ニューロン / 3次元培養 / 脱細胞組織 / 神経細胞シート / 神経シート / 脱細胞脳 / 長期培養 / 薬効評価 / ヒト由来神経細胞 |
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| Outline of Final Research Achievements |
This study aimed to develop the 3-dimensional culture techniques using decellualized brain tissue and layered neuronal sheet in order to construct a biological brain tissue model. We confirmed that human iPSC-derived neuronal networks were cultured on the prepared decellularized pig brain tissue. We also found that a solution of decellularized brain tissue promote the survival and the elongation of neurites in cultured human iPS cell-derived neurons. These results suggest that the culture method using decellularized tissue are useful to 3-dimensional brain tissue model. Furthermore, we found that hiPSC-derived neuron sheet with maintain function can be detached and layered using temperature sensitive substrate.
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Report
(4 results)
Research Products
(13 results)
