in vito reconstruction of 3-dimensional brain tissue using human cells

• Project/Area Number: 26560247
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Tohoku Institute of Technology
• Principal Investigator: Suzuki Ikuro 東北工業大学, 大学院工学研究科, 准教授 (90516311)
• Research Collaborator: KIMURA Tsuyosi
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: ヒトiPS細胞由来ニューロン / 3次元培養 / 脱細胞組織 / 神経細胞シート / 神経シート / 脱細胞脳 / 長期培養 / 薬効評価 / ヒト由来神経細胞

Outline of Final Research Achievements

This study aimed to develop the 3-dimensional culture techniques using decellualized brain tissue and layered neuronal sheet in order to construct a biological brain tissue model. We confirmed that human iPSC-derived neuronal networks were cultured on the prepared decellularized pig brain tissue. We also found that a solution of decellularized brain tissue promote the survival and the elongation of neurites in cultured human iPS cell-derived neurons. These results suggest that the culture method using decellularized tissue are useful to 3-dimensional brain tissue model. Furthermore, we found that hiPSC-derived neuron sheet with maintain function can be detached and layered using temperature sensitive substrate.

Research Products

• [Journal Article] Physiological maturation and drug responses of human induced pluripotent stem cell-derived cortical neuronal networks in long-term culture. (2016)
  • Author(s): Odawara A, Katoh H, Matsuda N, Suzuki I
  • Journal Title: Scientific Reports Volume: 6,26181 Issue: 1 Pages: 1-14
  • DOI: 10.1038/srep26181
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Induction of long-term potentiation and depression phenomena in human induced pluripotent stem cell-derived cortical neurons. (2016)
  • Author(s): Odawara A, Katoh H, Matsuda N, Suzuki I.
  • Journal Title: Biochem Biophys Res Commun. Volume: 469(4) Issue: 4 Pages: 856-62
  • DOI: 10.1016/j.bbrc.2015.12.087
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 創薬・再生医療への展開を目指したヒトiPS細胞由来神経ネットワークの機能評価技術 (2017)
  • Author(s): 鈴木郁郎
  • Organizer: 第16回 日本再生医療学会総会
  • Place of Presentation: 宮城
  • Year and Date: 2017-03-07
  • Invited
• [Presentation] 脱細胞化スライス脳を用いたin vitro三次元脳組織の再構築 (2017)
  • Author(s): 木村剛, 猪狩優花, 中村奈緒子, 橋本良秀, 藤里俊哉, 小田原あおい, 鈴木郁郎, 岸田晶夫
  • Organizer: 第16回 日本再生医療学会総会
  • Place of Presentation: 宮城
  • Year and Date: 2017-03-07
• [Presentation] Reconstruction of brain circuit using decellularized brain tissue (2016)
  • Author(s): Ikuro Suzuki, Aoi Odawara, Yuuka Igari, Naoko Nakamura, Akio Kishida, Tsuyoshi Kimura
  • Organizer: 第１回生体医歯工学共同研究拠点国際シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2016-11-10
• [Presentation] In vitro 脳再構築を目指した脳ECMでの神経細胞培養 (2016)
  • Author(s): 木村剛, 猪狩優花, 中村奈緒子, 橋本良秀, 藤里俊哉, 鈴木郁郎, 岸田晶夫
  • Organizer: 第３７回日本炎症・再生医学会
  • Place of Presentation: 京都
  • Year and Date: 2016-06-16
• [Presentation] Induction of long-term potentiation and depression phenomena in hiPSC-derived cortical neuronal networks. (2016)
  • Author(s): A. Odawara, H. Katoh, N. Matsuda, I. Suzuki.
  • Organizer: ISSCR International Symposium 2016
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2016-03-22
  • Int'l Joint Research
• [Presentation] Electrophysiological maturation and drug responses in long-term culture of hiPSC-derived cortical neuronal networks. (2016)
  • Author(s): I. Suzuki. A. Odawara, H. Katoh, N. Matsuda
  • Organizer: ISSCR International Symposium 2016
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2016-03-22
  • Int'l Joint Research
• [Presentation] Pharmacological Responses in Cultured Human iPSC Derived Cortical Neurons Using Multi-Electrode Array. (2016)
  • Author(s): A. Odawara, H. Katoh, N. Matsuda, Y. Shi, Ryan Arant, H. Jiko, I. Suzuki.
  • Organizer: Society of Toxicology
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2016-03-13
  • Int'l Joint Research
• [Presentation] Long-term electrophysiological activities and drug responses in cultured human iPSC derived neurons. (2015)
  • Author(s): A. Odawara, Y. Shi, H. Jiko, I. Suzuki.
  • Organizer: Neuroscience 2015, Society for neuroscience
  • Place of Presentation: Chicago, USA
  • Year and Date: 2015-10-17
  • Int'l Joint Research
• [Presentation] In vitro electrophysiological drug testing using human induced pluripotent stem cell-derived neurons. (2015)
  • Author(s): I. Suzuki, A. Odawara, Y. Shi, H. Jiko.
  • Organizer: ISSCR 2015, 13th Annual Meeting.
  • Place of Presentation: Stockholm, Sweden
  • Year and Date: 2015-06-24
  • Int'l Joint Research
• [Book] 特集：創薬とバイオマテリアル ～現状の組織・臓器別モデルと新しい三次元組織構築技術の応用～ 「ヒトiPS細胞由来ニューロンの長期培養と薬効評価技術」 (2015)
  • Author(s): 鈴木郁郎
  • Total Pages: 8
  • Publisher: 日本バイオマテリアル学会
• [Remarks] ヒトiPS細胞由来大脳皮質ニューロンの長期活動計測と電気生理学的な薬剤応答の検出に成功
  • URL: http://www.eis.tohtech.ac.jp/topics/20160519-01.html