| Project/Area Number |
26560250
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
OYANE Ayako 国立研究開発法人産業技術総合研究所, ナノ材料研究部門, 主任研究員 (50356672)
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| Co-Investigator(Renkei-kenkyūsha) |
KIKUCHI Masanori 物質・材料研究機構, 機能性材料研究拠点, グループリーダー (00354267)
WANG Xiupeng 産業技術総合研究所, 健康工学研究部門, 主任研究員 (70598789)
ITO Atsuo 産業技術総合研究所, 健康工学研究部門, グループリーダー (30356480)
YOSHINAKA Kiyoshi 産業技術総合研究所, 健康工学研究部門, 主任研究員 (90358341)
TSURUSHIMA Hideo 筑波大学, 医学医療系, 准教授 (50315470)
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| Research Collaborator |
ARAKI Hiroko 産業技術総合研究所, ナノ材料研究部門, テクニカルスタッフ
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
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| Keywords | DNA / リン酸カルシウム / 医療用輸液 / 遺伝子導入 / トランスフェクション / 細胞足場 / ナノコンポジット / 粒子 / サブミクロン / ＤＮＡ |
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| Outline of Final Research Achievements |
Calcium phosphate (CaP) layers immobilizing DNA (DNA-CaP composite layers) would be useful as surface components of tissue engineering scaffolds. This is because DNA-CaP composite layers possess a biocompatible surface suitable for cell growth and can control the cell behavior (differentiation, proliferation, etc.) through surface-mediated gene delivery. In this study, we fabricated DNA-CaP composite layers via coprecipitation in supersaturated CaP solutions that are prepared from infusion fluids. We shed light on the processes of coprecipitation and formation of the DNA-CaP composite layers in the supersaturated CaP solutions. Furthermore, we varied the coprecipitation conditions, i.e., coprecipitation time and Ca and P concentrations in solution, and obtained a guideline for the fabrication of DNA-CaP composite layers with high gene delivery capability and good cell compatibility.
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