| Project/Area Number |
26560400
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied health science
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| Research Institution | University of Shizuoka |
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Principal Investigator |
Miura Shinji 静岡県立大学, 食品栄養科学部, 教授 (10342932)
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| Co-Investigator(Kenkyū-buntansha) |
MORITA Akihito 静岡県立大学, 食品栄養科学部, 助教 (40239653)
IKEDA Masahiko 常葉大学, 環境学研究科, 教授 (20232176)
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| Co-Investigator(Renkei-kenkyūsha) |
GODA Toshinao 静岡県立大学, 食品栄養科学部, 教授 (70195923)
KAMEI Yasutomi 京都府立大学, 生命環境科学研究科, 教授 (70300829)
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| Research Collaborator |
SHIMBA Yuki
TOGAWA Hanako
SENOO Nanami
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 動脈硬化 / 筋線維の遅筋化 / 転写調節因子 / ApoE KOマウス / PGC-1α / FOXO1 / 健康・スポーツ科学 / 応用健康科学 / 生活習慣病 |
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| Outline of Final Research Achievements |
To examine whether the overexpression of PGC-1alpha in skeletal muscle could prevent the development of aortic atherosclerosis, we created the ApoE knockout mice overexpressing PGC-1alpha in the skeletal muscle. ApoE knockout mice without overexpression of PGC-1alpha were used as control. Hearts and fasting plasma samples were collected at the age of 16-20 weeks. The HE stained sections of the proximal aorta were evaluated for the cross-sectional area of lesions from the aortic root. Lipoprotein profiles were also measured. The skeletal muscle overexpression of PGC-1alpha could decrease by 43% in cross-sectional area of lesion as compared to control ApoE knockout mice. Plasma total cholesterol and VLDL-cholesterol levels were also decreased in ApoE knockout mice overexpressing PGC-1alpha.
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