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Analysis of p130Cas-mediated regulation of glucose metabolism

Research Project

Project/Area Number 26560410
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Applied health science
Research InstitutionNadogaya Hospital (Laboratory for Mechanical Medicine, Nadogaya Research Institute)

Principal Investigator

Sawada Yasuhiro  社会医療法人社団蛍水会名戸ヶ谷病院(名戸ヶ谷研究所メカノメディスン部門), 名戸ヶ谷研究所メカノメディスン部門, 部門長 (50313135)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsメカノトランスダクション / メカノバイオロジー / 糖代謝
Outline of Final Research Achievements

We generated muscle-, endothelium-, and osteocyte-specific p130Cas knockout (KO) mice by crossing floxed-p130Cas mice with transgenic mice specifically expressing Cre-recombinase in those individual tissues. Since none of these conditional p130Cas KO mice was embryonic lethal, we could analyze their phenotypes at an adult age (10 weeks after birth). Osteocyte-specific p130Cas KO mice exhibited apparently altered bone metabolism. In endothelium-specific p130Cas KO mice, vascular network formation appeared abnormal. We are currently investigating the molecular mechanism behind those phenotypes with specific reference to responses to mechanical perturbations. In contrast, no obvious phenotype was observed in muscle-specific p130Cas KO mice, which showed no difference from their control mice at glucose tolerance test, either with normal diet or after 12-week high fat diet. However, we have noted a possible role of the interstitium in glycolipid metabolism.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (13 results)

All 2016 2015 2014

All Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (8 results) (of which Int'l Joint Research: 3 results,  Invited: 5 results)

  • [Journal Article] Zbtb20 promotes astrocytogenesis during neocortical development2016

    • Author(s)
      Nagao, M., Ogata, T., Sawada, Y., Gotoh, Y.
    • Journal Title

      Nature Communications

      Volume: 7 Issue: 1 Pages: 11102-11102

    • DOI

      10.1038/ncomms11102

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Association between tensin 1 and p130Cas at focal adhesions links actin inward flux to cell migration2016

    • Author(s)
      Zhao Z, Tan SH, Machiyama H, Kawauchi K, Araki K, Hirata H, Sawada Y
    • Journal Title

      Biology Open

      Volume: 5 Issue: 4 Pages: 1-8

    • DOI

      10.1242/bio.016428

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 「緊張型」と「緩和型」のメカニカルストレスによる生体恒常性維持2015

    • Author(s)
      澤田泰宏, 原田伊知郎,市原克則, 吉野大輔, 吉村耕一
    • Journal Title

      実験医学

      Volume: 未確定

    • Related Report
      2014 Research-status Report
  • [Journal Article] Displacement of p130Cas from focal adhesions links actomyosin contraction to cell migration2014

    • Author(s)
      Machiyama H, Hirata H, Loh XK, Kanchi MM, Fujita H, Tan SH, Kawauchi K, Sawada Y
    • Journal Title

      Journal of Cell Science

      Volume: 127 Pages: 3440-3450

    • DOI

      10.1242/jcs.143438

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] 細胞-基質間接着のメカノトランスダクション2014

    • Author(s)
      澤田泰宏, 平田宏聡, 原田伊知郎.
    • Journal Title

      細胞工学

      Volume: 33 Pages: 950-954

    • Related Report
      2014 Research-status Report
  • [Presentation] 1生体のメカノセンシングを介する恒常性維持 -「善玉」メカニカルストレスと「悪玉」メカニカルストレス2016

    • Author(s)
      澤田泰宏
    • Organizer
      第43回日本生体電気・物理刺激研究会
    • Place of Presentation
      和歌山
    • Year and Date
      2016-03-05
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 3Mechanical Regulation and Maintenance of Organismal Homeostasis – Scientific Basis for the Health Promotion by Physical Motility and Exercise2015

    • Author(s)
      Sawada Y
    • Organizer
      The 2nd Congress, International Academy of Sportology
    • Place of Presentation
      東京
    • Year and Date
      2015-09-12
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 4メカニカルストレスによる生体機能制御 ― 恒常性維持に「善玉」あるいは「悪玉」として作用するメカニカルストレス2015

    • Author(s)
      澤田泰宏
    • Organizer
      第15回日本Men's Health医学会
    • Place of Presentation
      越谷
    • Year and Date
      2015-09-04
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Cell mechano-sensing and organismal homeostasis - Exploration of optimal exercise based on mechanobiology2015

    • Author(s)
      Sawada Y
    • Organizer
      International Symposium on Nanoarchitectonics for Mechanobiology
    • Place of Presentation
      筑波
    • Year and Date
      2015-07-29
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Why is ‘no mechanical stress’ bad for bone?2015

    • Author(s)
      Sawada Y
    • Organizer
      The 13th Congress of the International Society of Bone Morphometry
    • Place of Presentation
      東京
    • Year and Date
      2015-04-29
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Why is ‘no mechanical stress’ bad for bone?2015

    • Author(s)
      Sawada Y
    • Organizer
      The 13th Congress of the International Society of Bone Morphometry.
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2015-04-29
    • Related Report
      2014 Research-status Report
  • [Presentation] 細胞によるメカニカルストレス感知とこれを介する生体恒常性の維持 ー「適度な運動」の分子機構の探求2015

    • Author(s)
      澤田泰宏
    • Organizer
      早稲田大学基盤形成シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2015-03-07
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Temporal association of tensin1 and p130Cas (Cas) at focal adhesions serves as a molecular clutch that links inwardly moving actin cytoskeletons to cell migration2014

    • Author(s)
      Sawada Y, Tan SH, Zhao Z, Hirata H, Machiyama H
    • Organizer
      The 54th Annual Meeting of American Society for Cell Biology
    • Place of Presentation
      Philadelphia, USA
    • Year and Date
      2014-12-09
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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