Exploring RNA modifications using enzymatic probe and its applications
| Project/Area Number |
26560442
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemical biology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Suzuki Takeo 東京大学, 工学(系)研究科(研究院), 講師 (90533125)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | RNA修飾 / 修飾酵素 / 特異的標識 / 網羅的探索 / RNA / 化学標識 / 化学修飾 |
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| Outline of Final Research Achievements |
We aimed to explore transcriptome-wide RNA modifications with new techniques using covalent complexes between RNA modification and RNA modifying enzyme. We transiently expressed a modifying enzyme of interest in HeLa cells that were cultured under supplying a nucleic acid analogue molecule and could collect bound RNA from immune-precipitated RNA-enzyme complex in the analogue molecule dependent manner. Moreover, we examined a novel RNA modification labeling method based on enzymatic conversion of specific RNA modifications followed by nucleophilic substitution reaction. The method may provide a new solution to detect those specific modifications.
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Report
(3 results)
Research Products
(16 results)
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[Journal Article] A single methylation of 23S rRNA triggers late steps of 50S ribosomal subunit assembly2015
Author(s)
Arai, T., Ishiguro, K., Kimura, S., Sakaguchi, Y., Suzuki, T. and Suzuki, T.
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Journal Title
Proc. Natl. Acad. Sci. USA.
Volume: 112
Issue: 34
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Cdk5rap1-mediated 2-methylthio modification of mitochondrial tRNAs governs protein translation and contributes to myopathy in mice and humans.2015
Author(s)
Wei FY, Zhou B, Suzuki T, Miyata K, Ujihara Y, Horiguchi H, Takahashi N, Xie P, Michiue H, Fujimura A, Kaitsuka T, Matsui H, Koga Y, Mohri S, Suzuki T, Oike Y, Tomizawa K.
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Journal Title
Cell Metab
Volume: 21
Issue: 3
Pages: 413-6
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] A single acetylation of 18S rRNA is essential for biogenesis of the small ribosomal subunit in Saccharomyces cerevisiae2014
Author(s)
Ito, S., Akamatsu, Y., Noma, A., Kimura, S., Miyauchi, K., Ikeuchi, Y., Suzuki, T. and Suzuki, T
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Journal Title
J. Biol. Chem.
Volume: 289
Issue: 38
Pages: 26201-26212
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Presentation] Biogenesis of 5-taurinomethyluridine in human mitochondrial tRNAs; implication for molecular pathogenesis of mitochondrial diseases2015
Author(s)
Kana Asano, Takeo Suzuki, Fan-Yan Wei, Yoshiho Ikeuchi, Yuka Yashiro, Christin Tischner, Annette Hofer, Yoshihito Kishita, Yasushi Okazaki, Tina Wenz, Kazuhito Tomizawa, Tsutomu Suzuki
Organizer
第17回日本RNA学会年会
Place of Presentation
ホテルライフォート札幌, 札幌市, 北海道
Year and Date
2015-07-15
Related Report
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