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Study of high throughput screening based on chemical imaging sensor

Research Project

Project/Area Number 26620113
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Analytical chemistry
Research InstitutionSaitama University

Principal Investigator

UCHIDA Hidekazu  埼玉大学, 理工学研究科, 教授 (60223559)

Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords二次元電気化学センサ / LAAS / マイクロビーズ / アルコール脱水素酵素 / ホメピゾール / スクリーニング / 電気化学イメージセンサ / 酸化還元電流 / 酵素 / 電気化学センサ / 化学イメージングセンサ / アンペロメトリ / 酵素センサ
Outline of Final Research Achievements

We proposed the screening system based on the Light Addressable Amperometric Sensor (LAAS) which enables high throughput screening for drag discovery. The system can image a distribution of enzyme reaction under an optical microscope. We measured enzyme activity of microbeads spread between a LAAS sensor and a gel electrolyte. The enzyme modified microbeads that treated with fomepizole (inhibitor) can be discriminated from untreated one. We confirmed that the system can evaluate the efficacy of the inhibitor. There is the possibility of the drag screening on biological cells because the size of the microbead close to a cell.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (4 results)

All 2017 2016 2015

All Presentation (4 results)

  • [Presentation] 二次元電気化学センサ“LAAS”を用いた水溶液中におけるイオン拡散のリアルタイム観測2017

    • Author(s)
      尾崎拓真, 長谷川有貴, 内田秀和
    • Organizer
      電気学会 全国大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 二次元電気化学測定システムによる酵素固定化マイクロビーズ測定の研究2016

    • Author(s)
      下山千紘, 長谷川有貴, 内田秀和
    • Organizer
      電気学会E部門ケミカルセンサ研究会
    • Place of Presentation
      金沢市文化ホール(石川県・金沢市)
    • Year and Date
      2016-06-29
    • Related Report
      2016 Research-status Report
  • [Presentation] 有機半導体を用いた二次元電気化学センサデバイスの研究2015

    • Author(s)
      小澤諒平,長谷川有貴,内田秀和,早瀬貴文
    • Organizer
      電気学会
    • Place of Presentation
      福岡(九州大学医学部百年講堂)
    • Year and Date
      2015-07-02
    • Related Report
      2015 Research-status Report
  • [Presentation] 二次元電気化学センサの測定技術の研究2015

    • Author(s)
      野平充彦,長谷川有貴,内田秀和
    • Organizer
      電気学会
    • Place of Presentation
      福岡(九州大学医学部百年講堂)
    • Year and Date
      2015-07-02
    • Related Report
      2015 Research-status Report

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Published: 2014-04-04   Modified: 2019-03-29  

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