Study of high throughput screening based on chemical imaging sensor
Project/Area Number |
26620113
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Analytical chemistry
|
Research Institution | Saitama University |
Principal Investigator |
|
Project Period (FY) |
2014-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | 二次元電気化学センサ / LAAS / マイクロビーズ / アルコール脱水素酵素 / ホメピゾール / スクリーニング / 電気化学イメージセンサ / 酸化還元電流 / 酵素 / 電気化学センサ / 化学イメージングセンサ / アンペロメトリ / 酵素センサ |
Outline of Final Research Achievements |
We proposed the screening system based on the Light Addressable Amperometric Sensor (LAAS) which enables high throughput screening for drag discovery. The system can image a distribution of enzyme reaction under an optical microscope. We measured enzyme activity of microbeads spread between a LAAS sensor and a gel electrolyte. The enzyme modified microbeads that treated with fomepizole (inhibitor) can be discriminated from untreated one. We confirmed that the system can evaluate the efficacy of the inhibitor. There is the possibility of the drag screening on biological cells because the size of the microbead close to a cell.
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Report
(5 results)
Research Products
(4 results)