Does enzymatic activity involve in mitochondrial movement?

• Project/Area Number: 26620135
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Bio-related chemistry
• Research Institution: Kyushu University
• Principal Investigator: Koshiba Takumi 九州大学, 理学(系)研究科(研究院), 准教授 (70403970)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: ミトコンドリア / 酵素活性 / GTPase / メタロプロテアーゼ

Outline of Final Research Achievements

Mitochondria are dynamic organelles that undergo continuous fusion and fission, and these processes are believe to be essential for physiologic function of organisms. Once their balances become to be disrupted, it has been revealed that much of unexpected influences accumulate on physiologic functions.
Our aims of the project is to elucidate their mystery of the mitochondrial shape, and try to understand the mechanism by a molecular level. In this study, we focused on the role of a mitochondrial GTPase, OPA1 and the regulatory molecule of metalloproteases. We identified that OPA1 processing by the proteases would be an essential process for the maintaining of mitochondrial morphology.

Research Products

• [Journal Article] Protein-protein interactions of mitochondrial-associated protein via bioluminescence resonance energy transfer (2015)
  • Author(s): Koshiba, T.
  • Journal Title: Biophysics and Physicobiology Volume: 12 Pages: 31-35
  • NAID: 130005098799
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 抗ウイルス自然免疫におけるミトコンドリアの役割 (2015)
  • Author(s): 小柴琢己
  • Journal Title: 細胞工学 Volume: 34 Pages: 571-575
• [Journal Article] A型インフルエンザウイルスタンパク質PB1-F2とミトコンドリアとの相互作用 (2015)
  • Author(s): 吉住拓馬、小柴琢己
  • Journal Title: 生化学 Volume: 87 Pages: 144-148
  • NAID: 40020383817
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Loss of Miro1-directed retrograde mitochondrial movement results in a novel murine model for neuron disease (2014)
  • Author(s): Nguyen, T.T., Oh, S.S., Weaver, D., Lewandowska, A., Maxfield, D., Schuler, M.H., Smith, N.K., Macfarlane, J., Saunders, G., Palmer, C.A., Debattisti, V., Koshiba, T., Pulst, S., Feldman, E.L., Hajnóczky, G., and Shaw, J.M.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 111 Issue: 35
  • DOI: 10.1073/pnas.1402449111
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Influenza A virus protein PB1-F2 translocates into mitochondria via Tom40 channels and impairs innate immunity (2014)
  • Author(s): Yoshizumi, T., Ichinohe, T., Sasaki, O., Otera, H., Kawabata, S., Mihara, K., and #Koshiba, T.
  • Journal Title: Nature Communications Volume: 5 Issue: 1 Pages: 4713-4713
  • DOI: 10.1038/ncomms5713
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Mitochondria and antiviral signaling (2015)
  • Author(s): 小柴琢己
  • Organizer: BMB2015
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2015-12-01
  • Invited
• [Presentation] Influenza A viral protein PB1-F2 and mitochondria (2015)
  • Author(s): Takumi Koshiba
  • Organizer: The 5th Annual World Congress of Molecular & Cell Biology (CMCB-2015)
  • Place of Presentation: 中国・南京
  • Year and Date: 2015-04-25
  • Int'l Joint Research / Invited
• [Presentation] ミトコンドリアと抗RNAウイルス自然免疫 (2014)
  • Author(s): 小柴琢己
  • Organizer: 第52回 日本生物物理学会年
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2014-09-25 – 2014-09-27
  • Invited
• [Remarks]
  • URL: http://www.biology.kyushu-u.ac.jp/~koshiba/
• [Remarks]
  • URL: http://hyoka.ofc.kyushu-u.ac.jp/search/details/K002718/
• [Remarks] 九州大学研究者情報
  • URL: http://hyoka.ofc.kyushu-u.ac.jp/search/details/K002718/
• [Remarks] 九州大学大学院理学研究院生物科学部門統合生物学講座
  • URL: http://www.biology.kyushu-u.ac.jp/~koshiba/index.html