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Development of a novel cell separation method using both AC voltage and DC voltage

Research Project

Project/Area Number 26630417
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biofunction/Bioprocess
Research InstitutionGunma University

Principal Investigator

HAKODA Masaru  群馬大学, 大学院理工学府, 准教授 (00302456)

Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords細胞分離 / 誘電泳動 / 電気泳動 / 直流重畳交流電圧 / 細胞分離法
Outline of Final Research Achievements

In this study, the separation characteristics of two kinds of cells by DC voltage offset / AC voltage using an insulating film type dielectrophoresis separator with various slits were evaluated. In this device, cells having different DEP characteristics were separated by adopting a structure in which only parallel plate electrodes and insulating porous membranes were arranged in a flow channel through which a cell suspension flows. The separation efficiency of this device was proved to be affected by the number of slits, slit width, AC frequency, AC voltage, offset voltage and membrane permeation flow rate.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (7 results)

All 2018 2017 2016 2015

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Acknowledgement Compliant: 1 results) Presentation (5 results)

  • [Journal Article] 誘電回転法による人工多能性幹細胞の分化評価2018

    • Author(s)
      桜庭一樹、脇坂嘉一、中島崇仁、箱田優
    • Journal Title

      静電気学会誌

      Volume: 42 Pages: 9-14

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of Dielectrophoresis Separator with an Insulating Porous Membrane Using DC-Offset AC Electric Fields2016

    • Author(s)
      Masaru Hakoda
    • Journal Title

      Biotechnol. Prog.

      Volume: 32 Issue: 5 Pages: 1292-1300

    • DOI

      10.1002/btpr.2330

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] エレクトロローテーションを用いたヒト癌細胞の同定2018

    • Author(s)
      関口 諄、桜庭 一樹、脇坂 嘉一、箱田 優
    • Organizer
      第20回化学工学会学生発表会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 誘電泳動浮揚法を用いた iPS 細胞の分化評価2018

    • Author(s)
      本多剛之、桜庭 一樹、中島 崇仁、箱田 優
    • Organizer
      化学とマイクロナノシステム学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 誘電泳動現象を用いたiPS細胞の分化過程評価2017

    • Author(s)
      桜庭 一樹、脇坂 嘉一、中島 崇仁、箱田 優
    • Organizer
      化学とマイクロナノシステム学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 直流重畳交流電圧を用いたスリット付絶縁板型誘電泳動装置における連続分離特性2016

    • Author(s)
      一場 裕太, 北島 信義, 田島 創, 箱田 優
    • Organizer
      化学工学会 第81年会
    • Place of Presentation
      関西大学 千里山キャンパス
    • Year and Date
      2016-03-13
    • Related Report
      2015 Research-status Report
  • [Presentation] 直流重畳交流電圧を用いたスリット付絶縁板型誘電泳動2015

    • Author(s)
      一場 裕太, 古澤 尚也, 北島 信義, 田島 創, 箱田 優
    • Organizer
      化学工学会 第47回秋季大会
    • Place of Presentation
      北海道大学 札幌キャンパス
    • Year and Date
      2015-09-09
    • Related Report
      2015 Research-status Report

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Published: 2014-04-04   Modified: 2019-03-29  

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