| Project/Area Number |
26630418
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Teruyuki Nagamune 東京大学, 工学(系)研究科(研究院), 教授 (20124373)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 血中循環がん細胞単離技術 / 細胞光固定化技術 / 光活性化PEG脂質 / 血中循環細胞単離技術 / 血中循環がん細胞 / マイクロ流路 / 細胞アレイ / 光パターニング / 細胞回収技術 |
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| Outline of Final Research Achievements |
In this study, we aimed to develop the speedy and easy isolation method of blood circulation cancer cell (CTC) after detecting CTC by microscopic high content analysis of cells. For that purpose we developed the photoactivatable PEG-lipid reagent, which could immobilize cells on the surface of substrate by photoirradiation. By photoirradiating a specific cell on the reagent-modified surface and subsequent washing with buffer solution, photoirradiated cell was specifically and selectively immobilized on the surface. After seeding a sample derived from the peripheral blood of tumor bearing mouse in the microflow channel of which surface is modified with this reagent, we succeeded in isolating CTCs selectively inside the channel by detecting immunostained CTCs using fluorescent microscopy, immobilizing CTCs by photoirradiation and washout of non-photoirradiation cells.
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