Development of the method of cell-selective encapsulation in hydrogel sheaths

• Project/Area Number: 26630428
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biofunction/Bioprocess
• Research Institution: Osaka University
• Principal Investigator: Sakai Shinji 大阪大学, 基礎工学研究科, 准教授 (20359938)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 細胞包括 / 抗原抗体反応 / ヒドロゲル / 生体材料 / カプセル / 1細胞計測 / 細胞適合性

Outline of Final Research Achievements

We tried to develop a method realizing cell-selective encapsulation with a hydrogel sheath. The method is based on biospecific identification of the target cells through antigen-antibody reaction and subsequent enzymatic hydrogel sheath formation on the cell surfaces by horseradish peroxidase (HRP). The thickness of hydrogel sheath formed on cell surface was around 1 micrometer. The method was harmless for mammalian cells. In addition, the cells with and without the hydrogel sheath could be separate easily by incubating the cells on cell culture dish for several hours.

Research Products

• [Journal Article] Cell-selective encapsulation in hydrogel sheaths via biospecific identification and biochemical cross-linking (2015)
  • Author(s): Shinji Sakai, Yang Liu, Mikako Sengoku
  • Journal Title: Biomaterials Volume: 53 Pages: 494-501
  • DOI: 10.1016/j.biomaterials.2015.02.119
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] １細胞のヒドロゲル薄膜内包括による細胞識別、分離、制御 (2015)
  • Author(s): 境慎司
  • Organizer: 第38回日本分子生物学会年会／第88回日本生化学会大会 合同大会
  • Place of Presentation: 神戸国際会議業（兵庫県）
  • Year and Date: 2015-12-04
• [Presentation] ヒドロゲルによる細胞選択的な１細胞コーティング技術 (2015)
  • Author(s): 境慎司, 田谷正仁
  • Organizer: 第67回 日本生物工学会大会
  • Place of Presentation: 城山観光ホテル（鹿児島県）
  • Year and Date: 2015-10-26
• [Presentation] Individual cell-coating through on-cell surface enzymatic cross-linking (2015)
  • Author(s): Shinji Sakai
  • Organizer: The 3rd Annual Meeting of the Korean Society for Nanomedicine
  • Place of Presentation: Korea University Anam Hospital (Korea)
  • Year and Date: 2015-09-22
  • Int'l Joint Research / Invited
• [Presentation] Individual cell surface coating with a biocompatible thin hydrogel jacket (2015)
  • Author(s): Shinji Sakai, Masahito Taya
  • Organizer: ICMAT2015
  • Place of Presentation: Suntec Convention Center (Singapore)
  • Year and Date: 2015-06-08
  • Int'l Joint Research
• [Presentation] 細胞選択的ヒドロゲル内包括法の開発 (2015)
  • Author(s): 境 慎司， 仙石 未佳子， 劉 楊，田谷 正仁
  • Organizer: 化学工学会第80回年会
  • Place of Presentation: 芝浦工科大学（東京）
  • Year and Date: 2015-03-18 – 2015-03-20
• [Remarks] 選択的1細胞包括の論文がGlobal Medical Discoveryに掲載
  • URL: http://www.cheng.es.osaka-u.ac.jp/tayalabo/topics/1496.html
• [Remarks] 境准教授が韓国のナノメディシン学会で招待講演を行いました
  • URL: http://www.cheng.es.osaka-u.ac.jp/tayalabo/labtopics/1469.html
• [Remarks] Biomaterialsに論文が掲載されました
  • URL: http://www.cheng.es.osaka-u.ac.jp/tayalabo/labtopics/1358.html