Development of the method of cell-selective encapsulation in hydrogel sheaths
| Project/Area Number |
26630428
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Osaka University |
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Principal Investigator |
Sakai Shinji 大阪大学, 基礎工学研究科, 准教授 (20359938)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 細胞包括 / 抗原抗体反応 / ヒドロゲル / 生体材料 / カプセル / 1細胞計測 / 細胞適合性 |
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| Outline of Final Research Achievements |
We tried to develop a method realizing cell-selective encapsulation with a hydrogel sheath. The method is based on biospecific identification of the target cells through antigen-antibody reaction and subsequent enzymatic hydrogel sheath formation on the cell surfaces by horseradish peroxidase (HRP). The thickness of hydrogel sheath formed on cell surface was around 1 micrometer. The method was harmless for mammalian cells. In addition, the cells with and without the hydrogel sheath could be separate easily by incubating the cells on cell culture dish for several hours.
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Report
(3 results)
Research Products
(9 results)
