| Project/Area Number |
26640023
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurophysiology / General neuroscience
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
OCHIISHI TOMOYO 国立研究開発法人産業技術総合研究所, バイオメディカル研究部門, 主任研究員 (30356729)
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| Co-Investigator(Kenkyū-buntansha) |
KAKU Masami 茨城県立医療大学, 保健医療学部, 嘱託助手 (30646261)
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| Co-Investigator(Renkei-kenkyūsha) |
DOI Motomichi 国立研究開発法人産業技術総合研究所, バイオメディカル研究部門, 主任研究員 (50344213)
EBIHARA Tatsuhiko 国立研究開発法人産業技術総合研究所, バイオメディカル研究部門, 主任研究員 (00344119)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | アルツハイマー病 / アミロイドβタンパク質 / シナプス / オリゴマー / トランスジェニックマウス / GFP / 行動解析 / 長期増強 |
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| Outline of Final Research Achievements |
The intracellular accumulation of Amyloid-β (Aβ) oligomers critically contributes to disease progression in Alzheimer’s disease (AD) and can be contribute to the potential target of AD therapy. We developed Aβ-GFP fusion proteins that are oligomerized and visualize their dynamics inside cells. We also developed the Aβ-GFP transgenic mice that express Aβ-GFP fusion protein in neurons. The behavior analysis of this transgenic mice presented impaired retention memory compared with the non-transgenic mice in young age. These results suggest that the Aβ-GFP transgenic mouse is useful tool to investigate the toxicity of Aβ oligomer and pathogenic mechanisms of memory deficit of AD.
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