Development of new amyloid beta-GFP fusion protein which is a useful tool for the analysis of the synaptic function of amyloid beta monomers and oligomers
| Project/Area Number |
26640023
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurophysiology / General neuroscience
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
OCHIISHI TOMOYO 国立研究開発法人産業技術総合研究所, バイオメディカル研究部門, 主任研究員 (30356729)
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| Co-Investigator(Kenkyū-buntansha) |
KAKU Masami 茨城県立医療大学, 保健医療学部, 嘱託助手 (30646261)
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| Co-Investigator(Renkei-kenkyūsha) |
DOI Motomichi 国立研究開発法人産業技術総合研究所, バイオメディカル研究部門, 主任研究員 (50344213)
EBIHARA Tatsuhiko 国立研究開発法人産業技術総合研究所, バイオメディカル研究部門, 主任研究員 (00344119)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | アルツハイマー病 / アミロイドβタンパク質 / シナプス / オリゴマー / トランスジェニックマウス / GFP / 行動解析 / 長期増強 |
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| Outline of Final Research Achievements |
The intracellular accumulation of Amyloid-β (Aβ) oligomers critically contributes to disease progression in Alzheimer’s disease (AD) and can be contribute to the potential target of AD therapy. We developed Aβ-GFP fusion proteins that are oligomerized and visualize their dynamics inside cells. We also developed the Aβ-GFP transgenic mice that express Aβ-GFP fusion protein in neurons. The behavior analysis of this transgenic mice presented impaired retention memory compared with the non-transgenic mice in young age. These results suggest that the Aβ-GFP transgenic mouse is useful tool to investigate the toxicity of Aβ oligomer and pathogenic mechanisms of memory deficit of AD.
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Report
(3 results)
Research Products
(7 results)
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[Presentation] Development of new animal model of Alzheimer’s disease visualizing the intracellular dynamics of the amyloid-β protein.2015
Author(s)
Tomoyo Ochiishi, Motomichi Doi, Kazuhiko Yamasaki, Akira Kitamura, Takao Urabe, Nobutaka Hattori, Masataka Kinjo, Tatsuhiko Ebihara, Hideki Shimura
Organizer
Neuroscience 2015
Place of Presentation
Chicago, IL, USA
Year and Date
2015-10-17
Related Report
Int'l Joint Research
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