Generation of induction method of insulin producing cells from mouse liver cells in vivo

• Project/Area Number: 26640049
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Laboratory animal science
• Research Institution: University of Tsukuba
• Principal Investigator: Takahashi Satoru 筑波大学, 医学医療系, 教授 (50271896)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: インスリン / β細胞 / 再生 / ダイレクトリプログラミング / 肝細胞

Outline of Final Research Achievements

In order to develop a method that induces insulin-producing cells from the liver cells in living mice, we genarated conditinally over-expressing mice of Pdx1, NeuroD and MafA. Furthermore, We identifed a new factor that enhances the production of insulin from mouse liver cells with Pdx1, NeuroD and MafA. The new factor could enhance insulin transcription approximately 10-fold.

Research Products

• [Journal Article] Generation and characterization of MafA-Kusabira Orange mice. (2015)
  • Author(s): Nishimura W, Oishi H, Funahashi N, Fujiwara Takahashi S, Yasuda K.
  • Journal Title: Endocr J. Volume: 62(1) Pages: 37-51
  • NAID: 130004694063
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Pancreatic β-cell plasticity is regulated by maturation factor MafA. (2015)
  • Author(s): Nishimura W, Takahashi S, Yasuda K.
  • Journal Title: Diabetologica. Volume: 58(3) Pages: 566-574
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Role of Large MAF Transcription Factors in Mouse Endocrine Pancreas. (2015)
  • Author(s): Abdellatif AM, Ogata K, Kudo T, Xiafukaiti G, Chang Y-H, Kato MC, El-Morsy SE, Oishi H, Takahashi S.
  • Journal Title: Exp Animal. Volume: 64(3) Pages: 305-312
  • NAID: 130005091028
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Generation of insulin-producing cells from the mouse liver using β cell-related gene transfer including Mafa and Mafb. (2014)
  • Author(s): Nagasaki H, Katsumata T, Oishi H, Tai PH, Sekiguchi Y, Koshida R, Jung Y, Kudo T, Takahashi S.
  • Journal Title: PLoS One. Volume: 9(11)
  • Peer Reviewed / Open Access
• [Journal Article] MafA is required for postnatal proliferation of pancreatic β-cells. (2014)
  • Author(s): Eto K, Nishimura W, Oishi H, Udagawa H, Kawaguchi M, Hiramoto M, Fujiwara T, Takahashi S, Yasuda K.
  • Journal Title: PLoS One. Volume: 9(8)
  • Peer Reviewed / Open Access
• [Remarks] 解剖学・発生学研究室
  • URL: http://www.md.tsukuba.ac.jp/basic-med/anatomy/embryology/index.html