| Project/Area Number |
26640058
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory animal science
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
Ishida Yasumasa 奈良先端科学技術大学院大学, バイオサイエンス研究科, 准教授 (10221756)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 遺伝子トラップ / ＵＰＡＴｒａｐ / ＤＴｒａｐ / ジフテリア毒素 / 細胞系譜の除去 / ＥＳ細胞 / マウス / DTrap / 細胞系譜除去 / ハプロイドES細胞 / 両アレル遺伝子破壊 / テトラプロイド胚 |
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| Outline of Final Research Achievements |
When we perform gene-trapping experiments with mouse undifferentiated embryonic-stem (ES) cells by using a novel promoter-trapping strategy termed DTrap, in which the diphtheria-toxin (DT) gene is involved as a negative selectable marker, we are able to establish only the ES-cell clones whose transcriptionally silent genes had been trapped (disrupted). In the current research, we have tried to create the cell lineage-ablated mouse lines by performing complementation of mouse tetraploid embryos with such 'DTrapped' ES cells. We also aimed at rescuing the complemented tetraploid embryos by co-injecting the second type of wild-type or mutant ES cells.
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