| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
|
|---|
| Outline of Final Research Achievements |
Use of consomic mice played an important role to identify the responsible gene of the multiple factor disease. However, it was a problem that several years and a lot of labor for the manufacture process have been needed. In this study, I aimed to generate rapid system for generation of consomic mice by constructing the consomic cells in vitro via chromosome transfer and elimination technologies. Using whole cell fusion and chromosome transfer technique, I succeeded in introducing a B6-deribed chromosome 11 into 129-deribed mouse ES cells in which the 129-deribed chromosome 11 can be eliminated. The system will be expected to be a useful tool for the generation of consomic mice rapidly.
|
