Establishment and clinical application of the high sensitive effect assay of the HPV vaccine

• Project/Area Number: 26640096
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Tumor diagnostics
• Research Institution: Tokyo Metropolitan Institute of Medical Science
• Principal Investigator: ENDO Fumiko 公益財団法人東京都医学総合研究所, 生体分子先端研究分野, 研究員 (80546630)
• Co-Investigator(Kenkyū-buntansha): 芝崎 太 公益財団法人東京都医学総合研究所, ゲノム医科学研究分野, 分野長 (90300954)
• Co-Investigator(Renkei-kenkyūsha): TABATA Tsutomu 三重大学, 大学院・医学系研究科, 准教授 (40252358) ; KAWAMURA Machiko 埼玉県立がんセンター, 血液内科, 副部長 (80450592)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 子宮頸癌 / ワクチン / HPV / 子宮頸がんワクチン / 子宮頸がん予防ワクチン

Outline of Final Research Achievements

The cause of the cervical cancer onset is long-term infection to high risk type HPV (human papillomavirus), and it is said that, above all, a HPV16/18 type occupies 70%. The cervical cancer prevention vaccine spread widely all over the world, but did not establish the method to evaluate the effect objectively until now. In this study, we developed the method to measure a change of blood neutralization antibody titer of the HPV16/18 type with the vaccination in quickness and simpleness and easiness. At first we established the ELISA method using magnetic beads to reduce non-specific reaction and built the simple immunochromatography method which could judge one drop of serum next in 15 minutes. We succeeded in watching the agreement of the result of both methods from the result of the small clinical study of several hundred scales.

Research Products

• [Journal Article] Development of a Sinple and Quick Immunochromatography Method for Detection of anti-HPV-16/-18 Antibodies (2017)
  • Author(s): Fumiko Endo, Tsutomu Tabata, Daichi Sadato, Machiko Kawamura, Noriyuki Ando, Keisuke Oboki, Masako Ukaji, Kaoru Kobayashi, Yukuharu Kobayashi, Tomoaki Ikeda and Futoshi Shibasaki
  • Journal Title: PLoS One Volume: 12 Issue: 2 Pages: e0171314-e0171314
  • DOI: 10.1371/journal.pone.0171314
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] Development of the simple and easy immunochromatographic method for detecting the serum HPV-16/-18 antibodies (2015)
  • Author(s): 遠藤典子
  • Organizer: 第７４回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場（愛知県名古屋市）
  • Year and Date: 2015-10-08
• [Presentation] Development of the methods for HPV vaccine efficacy measurement (2014)
  • Author(s): 遠藤典子
  • Organizer: 第73回 日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（神奈川県）
  • Year and Date: 2014-09-25
• [Remarks] （公財）東京都医学総合研究所 ゲノム医科学研究分野 分子医療プロジェクト ホームページ
  • URL: http://www.molmed.jp/index.html