Genome-wide analysis of gene expression regulated by three dimensional chromatin structure

• Project/Area Number: 26640113
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Genome biology
• Research Institution: Kyoto University
• Principal Investigator: Sone Masamitsu 京都大学, iPS細胞研究所, 特定研究員 (90599771)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: クロマチン高次構造 / エンハンサー / エンハンサートラップ / 転写制御 / 3C / 多能性幹細胞 / 分化

Outline of Final Research Achievements

All the cells constituting our bodies posses complete genetic information that is encoded by DNA within the nuclei. Different cell types (i.e. muscle cells and neural cells) establish their own identities by selectively expressing different subsets of genes. Enhancers are DNA elements that interact with promoter regions of genes by looping from distant area to activate them. In this study, we tried to develop a method to globally analyze interactions between enhancers and genes. In future, using this method, we may elucidate the mechanism how enhancers selectively activate the gene expression in different cell types.