| Project/Area Number |
26640135
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
System genome science
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Chikara Furusawa 国立研究開発法人理化学研究所, 生命システム研究センター, チームリーダー (00372631)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 変動環境 / 進化実験 / 大腸菌 / 生体リズム現象 |
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| Outline of Final Research Achievements |
In this project, we analyzed evolutionary dynamics of E. coli under dynamically changing environment by using laboratory evolution. We cultured E. coli cells under periodically changing culture environments (e.g., carbon sources and addition of antibiotics) to obtain adapted cells to such dynamic environments. As results, we found that by adding two antibiotics periodically, resistance acquisition to one drug was significantly suppressed in comparison with stationary drug addition, while resistance acquisition to the other drug was unchanged. The results suggested that evolutionary routes can be biased by changing environment over time.
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