Development of structure-based Nanocapsule using vault particles.

• Project/Area Number: 26650020
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Structural biochemistry
• Research Institution: Osaka University
• Principal Investigator: TANAKA HIDEAKI 大阪大学, たんぱく質研究所, 准教授 (40346169)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
• Keywords: ボルト / ナノカプセル / Ｘ線結晶構造解析 / ドラッグデリバリーシステム / X線結晶構造解析

Outline of Final Research Achievements

Vaults are large barrel-shaped ribonucleoprotein particles and are found in numerous eukaryotic species. A vault particle shell is composed of 78 MVP (Major Vault Protein) chains with 39-fold dihedral symmetry, and has a large cavity. In this study, I developed novel Nanocapsules using vault particles based on its structural information.

Research Products

• [Presentation] Toward a high resolution structure determination of the vault (2015)
  • Author(s): Hideaki Tanaka
  • Organizer: International Symposium on Structure and Folding of Disease Related Proteins
  • Place of Presentation: Seoul National University, Korea
  • Year and Date: 2015-12-04
  • Int'l Joint Research / Invited
• [Presentation] レジストリ―の異なるダイニンストーク領域の二次構造解析 (2015)
  • Author(s): Haruka Iwasaki, Yosuke Nishikawa, Momoko Inatomi, Hideaki Tanaka, Genji Kurisu
  • Organizer: 第53回日本生物物理学会年会
  • Place of Presentation: 金沢大学角間キャンパス、石川県金沢市
  • Year and Date: 2015-09-13
• [Presentation] 光化学系I-フェレドキシンの電子伝達複合体構造から読み解く電子伝達の動的メカニズム (2015)
  • Author(s): 久保田（河合）寿子、武藤梨沙、Setif Pierre、Marc Nowaczyk 、Matthias Rogner、池上貴久、田中秀明、栗栖源嗣
  • Organizer: 第15回日本蛋白質科学会年会
  • Place of Presentation: あわぎんホール、徳島県徳島市
  • Year and Date: 2015-06-24
  • Invited
• [Presentation] Crystal Structure of Photosystem I from Synechocystis sp. PCC6803 at 5.1 A Resolution (2015)
  • Author(s): 河合(久保田)寿子、坂本由佳、和田元、田中秀明、栗栖源嗣
  • Organizer: 日本化学会第95春季年会
  • Place of Presentation: 日本大学 理工学部船橋キャンパス（千葉県船橋市）
  • Year and Date: 2015-03-26 – 2015-03-29
• [Presentation] 光化学系Iとフェレドキシンが形成する電子伝達複合体の構造基盤 (2014)
  • Author(s): 河合（久保田）寿子、武藤梨沙、池上貴久、Marc Nowaczyk、Matthias Rogner、田中秀明、栗栖源嗣
  • Organizer: 平成26年度日本結晶学会年会
  • Place of Presentation: 東京大学農学部（東京都文京区）
  • Year and Date: 2014-11-01 – 2014-11-03
• [Presentation] X-ray structure and NMR analysis of the electron transfer complex between photosystem I and ferredoxin (2014)
  • Author(s): Risa Mutoh, Hisako Kubota-Kawai, Marc Nowaczyk, Matthias Rogner, Hideaki Tanaka, Takahisa Ikegami, Genji Kurisu
  • Organizer: 第52回日本生物物理学会年会
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市）
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] 光化学系I－フェレドキシン複合体の構造解析 (2014)
  • Author(s): 武藤梨沙、久保田（河合）寿子、田中秀明、池上貴久、栗栖源嗣
  • Organizer: 第22回 「光合成セミナー2014：反応中心と色素系の多様性」
  • Place of Presentation: 名古屋工業大学（愛知県名古屋市）
  • Year and Date: 2014-07-12 – 2014-07-13
• [Patent(Industrial Property Rights)] Artificial Bioparticle and Method of Manufacturing the Same (2015)
  • Inventor(s): 田中秀明
  • Industrial Property Rights Holder: 田中秀明
  • Industrial Property Rights Type: 特許
  • Filing Date: 2015-03-23
  • Overseas