Vesicular dynamics in parietal and muscle cells

Research Project

Project/Area Number	26650034
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Functional biochemistry
Research Institution	Kyoto University
Principal Investigator	Takeshima Hiroshi 京都大学, 薬学研究科(研究院), 教授 (70212024)
Project Period (FY)	2014-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000) Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000) Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords	MG53 / TRIM50
Outline of Final Research Achievements	Mitsugumin 53 (MG53) is localized to repair vesicles underneath the cell membrane in muscle cells, and controls vesicular nucleation toward damaged sites during cell injury. TRIM50 is localized to tubulovesicles in in gastric parietal cells, and regulates vesicular dynamics during gastric acid secretion. MG53 and TRIM50 share a highly homologues structure, and we thus hypothesize that the repair vesicles and tubulovesicles may be regulated by similar molecular mechanisms. In this research project, we tried to identify structurally-similar components, for example, motor proteins and adaptor proteins to cytoskeleton, shared by muscular repair vesicles and parietal tubulovesicles.