Elucidation of the molecular mechanism of the pressure dependent fluorescence change of the YFP mutants toward the development of a pressure sensor.
| Project/Area Number |
26650036
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Functional biochemistry
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
Imada Katsumi 大阪大学, 理学(系)研究科(研究院), 教授 (40346143)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
WATANABE Nobuhisa 名古屋大学, シンクロトロン光研究センター, 教授 (70212321)
|
|---|
| Project Period (FY) |
2014-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Keywords | 蛍光タンパク質 / 結晶構造 / 圧力 / メカノバイオロジー / 生体エネルギー変換 / X線結晶構造解析 / 蛍光蛋白質 |
|---|
| Outline of Final Research Achievements |
Glycine insertion in β7 of YFP dramatically changes its fluorescence property dependent on hydrostatic pressure. In this study, we determined the crystal structures of the mutants and measured their fluorescence spectra under high pressure. We found the flipping of the carbonyl oxygen of the chromophore with the conformationl changes of the residues surrounding the chromophore under high pressure. These conformations are similar to those of YFP at normal pressure, suggesting these conformational changes cause the pressure response on the fluorescence property.
|
Report
(3 results)
Research Products
(6 results)
-
[Journal Article] Dependence of fluorescent protein brightness on protein concentration in solution and enhancement of it.2016
Author(s)
Takamitsu J. Morikawa, Hideaki Fujita, Akira Kitamura, Takashi Horio, Johtaro Yamamoto, Masataka Kinjo, Akira Sasaki, Hiroaki Machiyama, Keiko Yoshizawa, Taro Ichimura, Katsumi Imada, Takeharu Nagai & Tomonobu M. Watanabe
-
Journal Title
Scientific Reports
Volume: 6
Issue: 1
Pages: 22342-22342
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
