| Project/Area Number |
26650056
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Biophysics
|
|---|
| Research Institution | Institute of Physical and Chemical Research |
|---|
Principal Investigator |
Tanaka Motomasa 国立研究開発法人理化学研究所, 脳科学総合研究センター, チームリーダー (40321781)
|
|---|
| Research Collaborator |
Komi Yusuke 国立研究開発法人理化学研究所, 脳科学総合研究センター, 研究員 (20565999)
Shida Toshinobu 東京工業大学, 大学院・生命理工学研究科, 大学院生
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | 一分子 / タンパク質構造 / 力学計測 / 自閉症 / 蛋白質 / 一分子力学計測 / 酵母プリオン |
|---|
| Outline of Final Research Achievements |
Protein dynamically fluctuates in solution and adopts various distinct conformations. However, the measurement of such conformational heterogeneity of protein is technically difficult and the determination of a conformational space of protein is challenging. So far, single molecule force measurement with optical tweezers is a promising technique to address it, but it is not easily applied to nucleic acid proteins because the single protein molecule is tethered by a DNA handle linker which may alter a conformational space of the captured nucleic acid protein. In this study we developed a novel method of single molecule force measurement, using polyethylene glycol as a handle for single molecule of nucleic acid proteins.
|
