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Elucidation of mechanisms that regulate neural generation spatio-temporally in the brain using living embryos

Research Project

Project/Area Number 26650077
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Developmental biology
Research InstitutionKyoto University

Principal Investigator

Sehara-Fujisawa Atsuko  京都大学, 再生医科学研究所, 教授 (60209038)

Co-Investigator(Kenkyū-buntansha) SATO Fuminori  京都大学, 健康長寿社会の総合医療開発ユニット, 特定助教 (10588263)
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords発生・分化 / 組織・細胞 / 再生医学 / 生体分子 / 細胞・組織 / 細胞分化 / 増殖因子 / 神経分化 / ゼブラフィッシュ / ライブイメージング / ニューレグリン / ErbB
Outline of Final Research Achievements

Post-mitotic neurons are generated from neural progenitor cells (NPCs) at the expense of their proliferation. Live imaging revealed how production of post-mitotic neurons from neural progenitor cells is regulated in the sub-ventricular zone (SVZ). This neurogenesis depends on Neuregulin 1 type II (NRG1-II)-ErbB signaling. Moreover, we developed a novel fluorescent probe, which enables us to monitor the activity of the NRG1 ecotodomain shedding. Expression of the probe in zebrafish embryos revealed involvement of the NRG1 ecotodmain shedding in the spatio-temporal regulations of its signaling,.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (27 results)

All 2016 2015 2014

All Journal Article (6 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 6 results,  Open Access: 6 results) Presentation (20 results) (of which Int'l Joint Research: 3 results,  Invited: 5 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Prenatal regression of the trophotaenial placenta in a viviparous fish, Xenotoca eiseni2015

    • Author(s)
      Iida A, Nishimaki T, *Sehara-Fujisawa A
    • Journal Title

      Scientific Reports

      Volume: 5 Issue: 1 Pages: 7855-7855

    • DOI

      10.1038/srep07855

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Roles of ADAM8 in elimination of injured muscle fibers prior to skeletal muscle regeneration.2015

    • Author(s)
      Nishimura D, Sakai H, Sato T, Sato F, Nishimura S, Toyama-Sorimachi N, Bartsch JW, *Sehara-Fujisawa A.
    • Journal Title

      MECHANISMS OF DEVELOPMENT

      Volume: 135 Pages: 58-67

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Neuregulin 1 type II-ErbB signaling promotes cell divisions generating neurons from neural progenitor cells in the developing zebrafish brain2015

    • Author(s)
      Sato T, Sato F, Kamezaki A, Sakaguchi K, Tanigome R, Kawakami K, *Sehara-Fujisawa A
    • Journal Title

      PLOS ONE

      Volume: 10(5) Issue: 5 Pages: e0127360-e0127360

    • DOI

      10.1371/journal.pone.0127360

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] High-resolution live imaging reveals axon-glia interactions during peripheral nerve injury and repair in zebrafish2015

    • Author(s)
      Xiao Y, Faucherre A, Pola-Morell L, Heddleston J, Liu T, Chew T, Sato F, Sehara-Fujisawa A, Kawakami K, Lopez-Schier H
    • Journal Title

      Disease Models & Mechanisms

      Volume: 8 Issue: 6 Pages: 553-64

    • DOI

      10.1242/dmm.018184

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Must but not Mir-335 affects skeletal muscle growth and regeneration2015

    • Author(s)
      Hiramuki Y, Sato T, Furuta Y, Surani A, Sehara-Fujisawa A
    • Journal Title

      PLoS ONE

      Volume: 10 Issue: 6 Pages: e0130436-e0130436

    • DOI

      10.1371/journal.pone.0130436

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] miR-195/497 induce postnatal quiescence of skeletal muscle stem cells2014

    • Author(s)
      T. Sato, T. Yamamoto and A. Sehara-Fujisawa
    • Journal Title

      Nat Commun

      Volume: 5 Issue: 1 Pages: 4597-4597

    • DOI

      10.1038/ncomms5597

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Roles of ADAM8 in elimination of injured muscle fibers prior to skeletal muscle regeneration2016

    • Author(s)
      Nishimura D, Hiramuki Y, Sogabe M, Hori S, Sakai H, Sato T, Sato F, Bartsch JW, Sehara-Fujisawa A
    • Organizer
      CiRA/ISSCR 2016 International Symposia
    • Place of Presentation
      京都大学(京都府京都市)
    • Year and Date
      2016-03-22
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of a probe to monitor ectodomain shedding of Neureglin 1 in vitro and in vivo2016

    • Author(s)
      Kamezaki A, Sato F, Aoki K, Asakawa K, Kawakami K, Sehara-Fujisawa A
    • Organizer
      International FishMed Conference on Zebrafish Research 2016
    • Place of Presentation
      IIMCB(Warsaw, Poloand)
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 骨格筋維持機構の研究-再生医学から宇宙生物学まで2016

    • Author(s)
      瀬原淳子
    • Organizer
      京都大学同窓会セミナー
    • Place of Presentation
      チューリッヒ大学(スイス)
    • Year and Date
      2016-03-15
    • Related Report
      2015 Annual Research Report
  • [Presentation] 膜蛋白質のエクトドメイシェディングによる血管-神経相互作用の制御2016

    • Author(s)
      瀬原淳子
    • Organizer
      科学研究費助成事業新学術領域研究「血管と神経」成果公開シンポジウム
    • Place of Presentation
      京都大学(京都府京都市)
    • Year and Date
      2016-02-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] 膜型増殖因子の切断活性を可視化する蛍光プローブの開発とゼブラフィッシュ胚への応用2016

    • Author(s)
      亀崎青沙
    • Organizer
      第27回関西おさかな勉強会
    • Place of Presentation
      神戸大学(兵庫県神戸市)
    • Year and Date
      2016-01-15
    • Related Report
      2015 Annual Research Report
  • [Presentation] ニューレグリン-ErbBシグナルは脳室下帯において基底前駆細胞から神経細胞を生み出す分裂を促進する2015

    • Author(s)
      佐藤智美、佐藤文規、亀崎青沙、坂口和弥、谷米竜馬、梶原健、永島雅文、川上浩一、瀬原淳子
    • Organizer
      BMB2015
    • Place of Presentation
      神戸ポートピアホテル(神戸市)
    • Year and Date
      2015-12-04
    • Related Report
      2015 Annual Research Report
  • [Presentation] Prenatal regression of the trophotaenial placenta of the livebearer fish, Xenotoca eiseni2015

    • Author(s)
      Iida A, Nishimaki T, Sehara-Fujisawa A
    • Organizer
      BMB2015
    • Place of Presentation
      神戸ポートピアホテル(神戸市)
    • Year and Date
      2015-12-02
    • Related Report
      2015 Annual Research Report
  • [Presentation] Role of ADAM8 in elimination of injured muscle fibers prior to skeletal muscle regeneration2015

    • Author(s)
      Nishimura D, Sakai H, Sato T, Sato F, Nishimura S, Toyama-Sorimachi N, Bartsch JW, Sehara-Fujisawa A.
    • Organizer
      44th Europian Muscle Conference
    • Place of Presentation
      ワルシャワ大学(Warsaw, Poland)
    • Year and Date
      2015-09-22
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] NRG1-ErbB4 signaling promotes generation of neurons from neural progenitor cells in the developing brain.2015

    • Author(s)
      Sato T, Sato F, Kamezaki A, Sakaguchi K, Tanigome R, Kawakami K, Sehara A.
    • Organizer
      第58回日本神経化学会大会
    • Place of Presentation
      大宮ソニックシティ(埼玉県さいたま市)
    • Year and Date
      2015-09-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] What can we learn by exploring the interstitial space of skeletal muscle during development and regeneration?2015

    • Author(s)
      Sato F, Nishimura D, Hori S, Arai H, Hiramuki Y, Sogabe M, Kuriki M, Choi M, Wang Z, Kawahara A, Kawakami K, Sehara-Fujisawa A.
    • Organizer
      48th Annual meeting of the Japanese Society of Developmental Biologists
    • Place of Presentation
      つくば国際会議場(茨城県つくば市)
    • Year and Date
      2015-06-05
    • Related Report
      2015 Annual Research Report
  • [Presentation] A disintegrin and metalloprotease 12 (ADAM12) involves to the body growth at juveline stage in zebrafish2015

    • Author(s)
      Tokumasu Y, Iida A, Wang Z, Sehara-Fujisawa A.
    • Organizer
      48th Annual meeting of the Japanese Society of Developmental Biologists
    • Place of Presentation
      つくば国際会議場(茨城県つくば市)
    • Year and Date
      2015-06-04
    • Related Report
      2015 Annual Research Report
  • [Presentation] Real time imaging of Neuregulin 1 ectodomain-shedding in the developing zebrafish embryos2015

    • Author(s)
      Kamezaki A, Sato F, Aoki K, Kawakami K, Fukuhara S, Mochizuki N, Sehara-Fujisawa A.
    • Organizer
      48th Annual meeting of the Japanese Society of Developmental Biologists
    • Place of Presentation
      つくば国際会議場(茨城県つくば市)
    • Year and Date
      2015-06-03
    • Related Report
      2015 Annual Research Report
  • [Presentation] 細胞とゼブラフィッシュを用いた膜型タンパク質の切断のイメージング2015

    • Author(s)
      亀崎 青沙
    • Organizer
      第4回 Just do it! の会
    • Place of Presentation
      産業技術総合研究所つくばセンター(茨城県つくば市)
    • Year and Date
      2015-06-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] ゼブラフィッシュを用いた血管形成の研究2015

    • Author(s)
      王 梓
    • Organizer
      第4回 Just do it! の会
    • Place of Presentation
      産業技術総合研究所つくばセンター(茨城県つくば市)
    • Year and Date
      2015-06-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] Approach to spatio-temporal visualization of NRG1 ectodomain shedding in vitro and in vivo2015

    • Author(s)
      Aosa Kamezaki
    • Organizer
      Cell Biology, Developmental Biology, and Systems Biology Course Meeting
    • Place of Presentation
      京都大学医学部メモリアルホール(京都府京都市)
    • Year and Date
      2015-05-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] Mechanisms of Skeletal Muscle Regeneration2014

    • Author(s)
      Atsuko Sehara-Fujisawa
    • Organizer
      Symposium “Genomic and epigenomic insights into vertebrate regeneration, development and evolustion - development and evolution - Xenopus and fish as models”
    • Place of Presentation
      Pontificia Universidad Catolica de Chile(チリ・カトリカ大学)
    • Year and Date
      2014-11-05
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Novel Molecular and Cellular Mechanisms of Skeletal Muscle Regeneration2014

    • Author(s)
      Atsuko Sehara-Fujisawa
    • Organizer
      KEY Forum: From Stem Cells to Organs  熊本医学・生物科学国際シンポジウム「幹細胞制御と臓器再建」
    • Place of Presentation
      熊本市医師会館(熊本県熊本市)
    • Year and Date
      2014-09-05
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Mechanisms of Skeletal Muscle Regeneration2014

    • Author(s)
      Atsuko Sehara-Fujisawa
    • Organizer
      The 2nd Kyoto University & National Taiwan University Symposium 2014 -Biology-
    • Place of Presentation
      京都大学(京都府京都市)
    • Year and Date
      2014-09-01
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Exploring Mechanisms of Neurogenesis in the Developing Brain with Live Zebrafish Embryos2014

    • Author(s)
      Tomomi Sato, Fuminori Sato, Aosa Kamezaki, Koichi Kawakami, Atsuko Sehara-Fujisawa
    • Organizer
      第66回日本細胞生物学会大会 シンポジウム7「細胞生物学における小型魚類の魅力」
    • Place of Presentation
      奈良県新公会堂(奈良県奈良市)
    • Year and Date
      2014-06-12
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 神経細胞分化を制御する脳内細胞外環境2014

    • Author(s)
      瀬原淳子
    • Organizer
      第14回日本抗加齢医学会総会 Basic Science 4「抗加齢をめざす、細胞外環境による幹細胞維持・分化制御の探索」
    • Place of Presentation
      大阪国際会議場(大阪府大阪市)
    • Year and Date
      2014-06-08
    • Related Report
      2014 Research-status Report
    • Invited
  • [Patent(Industrial Property Rights)] 膜型ニューレグリンのエクトドメインシェディング可視化プローブ2016

    • Inventor(s)
      瀬原淳子・亀崎青沙・佐藤文規・青木一洋・川上浩一
    • Industrial Property Rights Holder
      瀬原淳子・亀崎青沙・佐藤文規・青木一洋・川上浩一
    • Industrial Property Rights Type
      特許
    • Filing Date
      2016-03-16
    • Related Report
      2015 Annual Research Report

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Published: 2014-04-04   Modified: 2017-05-10  

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