Development of forward gentics methods in appendicularians as a novel chordate model organism
| Project/Area Number |
26650079
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Developmental biology
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| Research Institution | Osaka University |
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Principal Investigator |
Nishida Hiroki 大阪大学, 理学(系)研究科(研究院), 教授 (60192689)
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| Co-Investigator(Kenkyū-buntansha) |
ONUMA Takeshi 大阪大学, 大学院理学研究科, 助教 (30632103)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | オヤマボヤ / 尾虫類 / 遺伝子導入 / DNAi / トランスジェネシス / RNAseq / 遺伝子機能阻害 / オタマボヤ / 脊索動物 / トランスジェニック / トランスポゾン / 突然変異体形成 / 胚発生 / エンハンサートラップ / 変異体形成 |
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| Outline of Final Research Achievements |
The appendicularian, Oikopleura dioica is a planktonic chordate, which share basic bodyplan with vertebrates. The life cycle is just 5 days. In this project, we aimed to make O. dioica an emerging model animal with which genetic approaches are feasible, such as in nematode and fly. In the processes of this project, we found that novel DNAi method works in this animal. Gene knockdown using DNAi will facilitate to screen and find developmentally important genes with ease and less expensive way.
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Report
(3 results)
Research Products
(33 results)
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[Journal Article] Guidelines for the nomenclature of genetic elements in tunicate genomes.2015
Author(s)
Stolfi, A., Sasakura, Y., Chalopin, D., Satou, Y., Christiaen, L., Dantec, C., Endo, T., Naville, M., Nishida, H., Swalla, B., Volff J-N, Voskoboynik, A., Dauga, D., and Lemaire, P.
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Journal Title
Genesis
Volume: 53
Issue: 1
Pages: 1-14
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] ANISEED 2015: a digital framework for the comparative developmental biology of ascidians.2015
Author(s)
Brozovic, Martin, Dantec, Dauga, Mendez, Simion, Percher, Laporte, Scornavacca, Di Gregorio, Fujiwara, et al.
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Journal Title
Nucleic Acids Research
Volume: 44
Issue: D1
Pages: 808-818
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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