Development of forward gentics methods in appendicularians as a novel chordate model organism

• Project/Area Number: 26650079
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Developmental biology
• Research Institution: Osaka University
• Principal Investigator: Nishida Hiroki 大阪大学, 理学(系)研究科(研究院), 教授 (60192689)
• Co-Investigator(Kenkyū-buntansha): ONUMA Takeshi 大阪大学, 大学院理学研究科, 助教 (30632103)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: オヤマボヤ / 尾虫類 / 遺伝子導入 / DNAi / トランスジェネシス / RNAseq / 遺伝子機能阻害 / オタマボヤ / 脊索動物 / トランスジェニック / トランスポゾン / 突然変異体形成 / 胚発生 / エンハンサートラップ / 変異体形成

Outline of Final Research Achievements

The appendicularian, Oikopleura dioica is a planktonic chordate, which share basic bodyplan with vertebrates. The life cycle is just 5 days. In this project, we aimed to make O. dioica an emerging model animal with which genetic approaches are feasible, such as in nematode and fly. In the processes of this project, we found that novel DNAi method works in this animal. Gene knockdown using DNAi will facilitate to screen and find developmentally important genes with ease and less expensive way.

Research Products

• [Int'l Joint Research] CRBM(フランス)
• [Journal Article] Guidelines for the nomenclature of genetic elements in tunicate genomes. (2015)
  • Author(s): Stolfi, A., Sasakura, Y., Chalopin, D., Satou, Y., Christiaen, L., Dantec, C., Endo, T., Naville, M., Nishida, H., Swalla, B., Volff J-N, Voskoboynik, A., Dauga, D., and Lemaire, P.
  • Journal Title: Genesis Volume: 53 Issue: 1 Pages: 1-14
  • DOI: 10.1002/dvg.22822
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] REGULATOR: a database of metazoan transcription factors and maternal factors for developmental studies. (2015)
  • Author(s): Wang, K., and Nishida, H.
  • Journal Title: BMC Bioinformatics Volume: 16 Issue: 1 Pages: 114-114
  • DOI: 10.1186/s12859-015-0552-x
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] DNA-induced gene silencing in the appendicularian Oikopleura dioica. (2015)
  • Author(s): Omotezako, T., Onuma, T. A., and Nishida, H.
  • Journal Title: Proc. R. Soc. B Volume: 282 Issue: 1807 Pages: 20150435-20150435
  • DOI: 10.1098/rspb.2015.0435
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Maternal and zygotic transcriptomes in the appendicularian, Oikopleura dioica: Novel protein-encoding genes, intra-species sequence variations, and trans-spliced RNA leader. (2015)
  • Author(s): Wang K., Omotezako T., Kishi K., Nishida H., and Onuma T. A.
  • Journal Title: Development Genes and Evolution Volume: 225(3) Issue: 3 Pages: 149-159
  • DOI: 10.1007/s00427-015-0502-7
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Polarization of PI3K activity initiated by ooplasmic segregation guides nuclear migration in the mesendoderm. (2015)
  • Author(s): Takatori, N., Oonuma, K., Nishida, H. and Saiga, H.
  • Journal Title: Developmental Cell Volume: 35 Issue: 3 Pages: 333-343
  • DOI: 10.1016/j.devcel.2015.10.012
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] ANISEED 2015: a digital framework for the comparative developmental biology of ascidians. (2015)
  • Author(s): Brozovic, Martin, Dantec, Dauga, Mendez, Simion, Percher, Laporte, Scornavacca, Di Gregorio, Fujiwara, et al.
  • Journal Title: Nucleic Acids Research Volume: 44 Issue: D1 Pages: 808-818
  • DOI: 10.1093/nar/gkv966
  • NAID: 120007129589
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] DNA interference: DNA-induced gene silencing in the appendicularian Oikopleura dioica. (2015)
  • Author(s): Omotezako, T., Onuma, T. A., and Nishida, H.
  • Journal Title: Proc. R. Soc. B Volume: in press
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Regulation of the number of cell division rounds by tissue-specific transcription factors and Cdk inhibitor during ascidian embryogenesis. (2014)
  • Author(s): Kuwajima, M., Kumano, G., and Nishida, H.
  • Journal Title: PLOS ONE Volume: 9 Issue: 3 Pages: e90188-e90188
  • DOI: 10.1371/journal.pone.0090188
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Transcription factor Tbx6 plays a central role in fate determination between mesenchyme and muscle in embryos of the ascidian, Halocynthia roretzi. (2014)
  • Author(s): Kumano, G., Negoro, N., and Nishida, H.
  • Journal Title: Dev. Growth Differ. Volume: 56 Issue: 4 Pages: 310-322
  • DOI: 10.1111/dgd.12133
  • NAID: 40020059339
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Control of the number of cell division rounds in distinct tissues during ascidian embryogenesis. (2014)
  • Author(s): Yamada, A., and Nishida, H.
  • Journal Title: Dev. Growth Differ. Volume: 56 Issue: 5 Pages: 376-386
  • DOI: 10.1111/dgd.12141
  • NAID: 40020109517
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Cell lineages and fate maps in tunicates: Conservation and modification (2014)
  • Author(s): Nishida, H., and Stach, T.
  • Journal Title: Zool. Sci. Volume: 31 Issue: 10 Pages: 645-652
  • DOI: 10.2108/zs140117
  • NAID: 40020231557
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Long-distance cell migrations during larval development in the appendicularian, Oikopleura dioica. (2014)
  • Author(s): Kanae Kishi, Takeshi A. Onuma, and Hiroki Nishida.
  • Journal Title: Developmental Biology Volume: 395(2) Issue: 2 Pages: 299-306
  • DOI: 10.1016/j.ydbio.2014.09.006
  • Peer Reviewed
• [Presentation] 脊索動物ワカレオタマボヤの表皮パターニングと系譜解析 (2015)
  • Author(s): 岸香苗，林桃子，小沼健，西田宏記
  • Organizer: 日本動物学会
  • Place of Presentation: 朱鷺メッセ（新潟）
  • Year and Date: 2015-09-17
• [Presentation] オタマボヤの左右対称な器官の形成は細胞系譜レベルの左右非対称性をもつ (2015)
  • Author(s): 小沼健，林桃子，西田宏記
  • Organizer: 日本動物学会
  • Place of Presentation: 朱鷺メッセ（新潟）
  • Year and Date: 2015-09-17
• [Presentation] オタマボヤを用いた母性と胚性のRNA-Seq (2015)
  • Author(s): 王凱，表迫竜也，岸香苗，西田宏記，小沼健
  • Organizer: 日本動物学会
  • Place of Presentation: 朱鷺メッセ（新潟）
  • Year and Date: 2015-09-17
• [Presentation] ワカレオタマボヤの甲状腺・内柱関連遺伝子群の発現 (2015)
  • Author(s): 平尾早智澄，Kai Wang，小沼健，西田宏記，小笠原道生
  • Organizer: 日本動物学会
  • Place of Presentation: 朱鷺メッセ（新潟）
  • Year and Date: 2015-09-17
• [Presentation] Oocyte maturation and establishment of the animal-vegetal axis in ascidians (2015)
  • Author(s): Masumi Tokuhisa, and Hiroki Nishida
  • Organizer: The 8th International Tunicate Meeting
  • Place of Presentation: Aomori City Cultural Hall （青森）
  • Year and Date: 2015-07-13
  • Int'l Joint Research
• [Presentation] Maternal and zygotic transcriptomes in the appendicularian, Oikopleura dioica: Novel protein-encoding genes, intra-species sequence variations, and trans-spliced RNA leader (2015)
  • Author(s): Kai Wang, Tatsuya Omotezako, Kanae Kishi, Hiroki Nishida, and Takeshi A. Onuma
  • Organizer: The 8th International Tunicate Meeting
  • Place of Presentation: Aomori City Cultural Hall （青森）
  • Year and Date: 2015-07-13
  • Int'l Joint Research
• [Presentation] Morphogenesis and patterning of the trunk epidermis of the appendicuralian, Oikopleura dioica (2015)
  • Author(s): Kanae Kishi, Momoko Hayashi, Takeshi A. Onuma, Hiroki Nishida
  • Organizer: The 8th International Tunicate Meeting
  • Place of Presentation: Aomori City Cultural Hall （青森）
  • Year and Date: 2015-07-13
  • Int'l Joint Research
• [Presentation] Internal and external morphology of adults of the appendicularian, Oikopleura dioica: A SEM study (2015)
  • Author(s): Takeshi A. Onuma, Miho Isobe, Hiroki Nishida
  • Organizer: The 8th International Tunicate Meeting
  • Place of Presentation: Aomori City Cultural Hall （青森）
  • Year and Date: 2015-07-13
  • Int'l Joint Research
• [Presentation] DNA interference: new gene silencing method in the appendicularian, Oikopleura dioica (2015)
  • Author(s): Tatsuya Omotezako, Takeshi A. Onuma, and Hiroki Nishida
  • Organizer: The 8th International Tunicate Meeting
  • Place of Presentation: Aomori City Cultural Hall （青森）
  • Year and Date: 2015-07-13
  • Int'l Joint Research
• [Presentation] Oocyte maturation and establishment of the animal-vegetal axis in ascidians (2015)
  • Author(s): Hiroki Nishida
  • Organizer: Oocyte maturation and Fertilization Meeting IV
  • Place of Presentation: 東北大学浅虫海洋生物学教育研究センター（青森）
  • Year and Date: 2015-06-15
  • Int'l Joint Research / Invited
• [Presentation] オタマボヤを用いた母性と胚性のRNA-Seq: 新規遺伝子、種内変異、トランススプライシング (2015)
  • Author(s): 王凱，表迫竜也，岸香苗，西田宏記，小沼健
  • Organizer: 日本発生生物学会
  • Place of Presentation: International Congress Center (筑波)
  • Year and Date: 2015-06-02
• [Presentation] 走査型電子顕微鏡による脊索動物ワカレオタマボヤの外部構造・内部構造の観察 (2015)
  • Author(s): 小沼健、磯部美穂、西田宏記
  • Organizer: 日本発生生物学会
  • Place of Presentation: International Congress Center (筑波)
  • Year and Date: 2015-06-02
• [Presentation] 脊索動物ワカレオタマボヤの表皮パターニングと系譜解析 (2015)
  • Author(s): 岸香苗、林桃子、小沼健、西田宏記
  • Organizer: 日本発生生物学会
  • Place of Presentation: International Congress Center (筑波)
  • Year and Date: 2015-06-02
• [Presentation] RNA interference and DNA interference: Two gene silencing systems in the appendicularian Oikopleura dioica (2014)
  • Author(s): 表迫竜也、小沼健、西田宏記
  • Organizer: 日本分子生物学会
  • Place of Presentation: パシフィコ横浜（横浜）
  • Year and Date: 2014-11-26
• [Presentation] オタマボヤを用いた発生額研究の近況 (2014)
  • Author(s): 小沼健、表迫竜也、岸香苗、西田宏記
  • Organizer: ホヤ研究会
  • Place of Presentation: 筑波大学東京キャンパス（東京）
  • Year and Date: 2014-10-13
• [Presentation] ワカレオタマボヤ幼生期における三種の長距離移動細胞の移動メカニズム (2014)
  • Author(s): 岸香苗、小沼健、西田宏記
  • Organizer: 日本動物学会
  • Place of Presentation: 東北大学川内北キャンパス（仙台）
  • Year and Date: 2014-09-11
• [Presentation] 脊索動物ワカレオタマボヤの左右非対称性の形成:２細胞期胚の左右割球の標識追跡 (2014)
  • Author(s): 林桃子、小沼健、西田宏記
  • Organizer: 日本動物学会
  • Place of Presentation: 東北大学川内北キャンパス（仙台）
  • Year and Date: 2014-09-11
• [Presentation] 脊索動物ワカレオタマボヤの走査型電子顕微鏡による観察 (2014)
  • Author(s): 小沼健、磯部美穂、西田宏記
  • Organizer: 日本動物学会
  • Place of Presentation: 東北大学川内北キャンパス（仙台）
  • Year and Date: 2014-09-11
• [Remarks] Hiroki NISHIDA's Laboratory
  • URL: http://www.bio.sci.osaka-u.ac.jp/bio_web/lab_page/nishida/index.html
• [Remarks] 西田研究室ホームページ
  • URL: http://www.bio.sci.osaka-u.ac.jp/bio_web/lab_page/nishida/index.html