Technological development of conditional knockout system in newts for investigation of cardiac regeneration
| Project/Area Number |
26650082
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Developmental biology
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| Research Institution | Tottori University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
Hayashi Toshinori 鳥取大学, 医学部, 准教授 (60580925)
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| Research Collaborator |
Satoh Yukio 鳥取大学, 医学部, 助教
Azuma Shouhei 鳥取大学, 大学院医学系研究科, 大学院生
Nakajima Mie 鳥取大学, 大学院医学系研究科, 大学院生
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | イベリアトゲイモリ / 心筋細胞 / 再生 / トランスジェニック / 細胞系譜 / コンディショナルノックアウト / CRE-loxP / IR-LEGO / 発生 |
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| Outline of Final Research Achievements |
In this research, we aimed to establish a research base to elucidate cardiac regeneration of the newt at the gene level and obtained the following results, and obtained the following results. (1) We tried to generate a conditional knockout newt of the CDK1 gene, showed that TALENs can efficiently cleave CDK1 allele, and that incomplete but loxP sequence can be inserted to the CDK1 allele. (2) Local induction of genes by IR-LEGO method was established. (3) Construction of gene expression database and efficient genomic cloning method. (4) The conditions of CRISPR / Cas 9 system in newts were determined.
Based on these results, we published 3 articles of the original paper and 2 reviews of the review. We also made 17 presentations at academic conferences.
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Report
(4 results)
Research Products
(45 results)
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[Journal Article] Two inhibitory systems and CKIs regulate cell cycle exit of mammalian cardiomyocytes after birth.2015
Author(s)
Tane, S., Okayama, H., Ikenishi, A., Amemiya, Y., Nakayama, I. K., and Takeuchi, T.
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Journal Title
Biochem. Biophys. Res. Commun.
Volume: 466
Issue: 2
Pages: 147-154
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Application of local gene induction by infrared laser-mediated microscope and temperature stimulator to amphibian regeneration study.2015
Author(s)
Kawasumi, A., Hayashi, T., Kobayashi, T., Nagayama, C., Hayashi, S., Kamei, Y., Morishita, Y., T Takeuchi, T., Tamura, K., Yokoyama, H.
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Journal Title
Dev. Growth Differ.
Volume: 57
Issue: 9
Pages: 601-613
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Repression of cyclin D1 expression is necessary for the maintenance of cell cycle exit in adult mammalian cardiomyocytes2014
Author(s)
Tane, S., Kubota, M., Okayama, H.,Ikenishi, A., Yoshitome, S., Iwamoto,N., Satoh, Y., Kusakabe, A., Ogawa, S.,Kanai, A., Molkentin, J. D., Nakamura, K., Ohbayashi, T., and Takeuchi, T.
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Journal Title
J. Biol. Chem
Volume: (in press)
Issue: 26
Pages: 18033-18044
DOI
Related Report
Peer Reviewed
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[Journal Article] CDK inhibitors, p21Cip1 and p27Kip1, participate in cell cycle exit of mammalian cardiomyocytes.2014
Author(s)
Tane, S., Ikenishi, A., Okayama, H., Iwamoto, N., Nakayama, I. K., and Takeuchi, T.
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Journal Title
Biochem. Biophys. Res. Commun.
Volume: 443
Issue: 3
Pages: 1105-1109
DOI
Related Report
Peer Reviewed
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[Journal Article] Transcription activator-like effector nucleases efficiently disrupt the target gene in Iberian ribbed newts (Pleurodeles waltl), an experimental model animal for regeneration.2014
Author(s)
Hayashi T, Sakamoto K, Sakuma T, Yokotani N, Inoue T, Kawaguchi E, Agata K, Yamamoto T, Takeuchi T
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Journal Title
Development, Growth and Differentiation
Volume: 56
Issue: 1
Pages: 115-121
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] What determines differences in cardiac regenerative abilities between mouse and newts?2014
Author(s)
Takeuchi, T., Tane, S., Okayama, Ikenishi, A., H., Amemiya Y., Ohdira, Y., Myouga, A., Azuma, S., Hayashi, T.
Organizer
日本分子生物学会 シンポジウム
Place of Presentation
パシフィコ横浜 横浜市
Year and Date
2014-11-25 – 2014-11-27
Related Report
Invited
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[Presentation] Genome editing is a powerful tool for studies in newt cardiac regeneration2014
Author(s)
Hayashi, T., Sakuma, T., Myouga, A., Sakamoto, K., Yokotani, N., Inoue, T., Kawaguchi, E., Agata, K., Yamamoto, T., and Takeuchi, T.
Organizer
日本遺伝学会大会 ワークショップ
Place of Presentation
長浜バイオ大学 長浜市
Year and Date
2014-09-17
Related Report
Invited
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[Presentation] Mechanisms of cell cycle exit and the maintenance in mouse cardiomyocytes, and the roles for regenerative abilities in mouse and newts.2014
Author(s)
Takeuchi, T., Tane, S., Okayama, Ikenishi, A., Amemiya, Y., Myouga, A., Hayashi, T.
Organizer
EMBO Conference The molecular &Cellular Basis of Regeneration & Tissue Repair,
Place of Presentation
Sant Feliu de Guixols, Spain.
Year and Date
2014-09-06 – 2014-09-10
Related Report
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[Presentation] Cell cycle exit in cardiomyocytes is initiated by p21Cip1 and p27Kip1, and maintained by two inhibitor systems.2014
Author(s)
Tane, S., Okayama, H., Ikenishi, A., Satoh, Y., Takeuchi, T.
Organizer
日本発生生物学会
Place of Presentation
ウィンク愛知 名古屋市
Year and Date
2014-05-27 – 2014-05-30
Related Report
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[Presentation] TALEN-mediated genome editing is very useful in Iberian ribbed newts (Pleurodeles waltl), an experimental model animal for regeneration.2014
Author(s)
Hayashi, T., Sakamoto, K., Sakuma, T., Myouga, A., Yokotani, N., Inoue, T., Kawaguchi, E, Agata, K., Yamamoto, T., Takeuchi, T.
Organizer
日本発生生物学会
Place of Presentation
ウィンク愛知 名古屋市
Year and Date
2014-05-27 – 2014-05-30
Related Report
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