Development of model system to understand nematode infection mechanisms.

• Project/Area Number: 26650102
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kumamoto University
• Principal Investigator: Sawa Shinichiro 熊本大学, 大学院先端科学研究部(理), 教授 (00315748)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 植物感染性線虫 / 感染機構 / M. incognita / A. thaliana / ネコブセンチュウ / サツマイモネコブセンチュウ / 誘引物質 / 巨大細胞 / センチュウ感染

Outline of Final Research Achievements

Developmental plasticity is one of the most striking features of plant morphogenesis, as plants are able to vary their shapes in response to environmental cues. Root-knot nematodes (RKNs) are known to parasitize multiple species of rooting plants and to induce characteristic tissue expansion called galls or root-knots on the roots of their hosts by perturbing the plant cellular machinery.
In this study, we deciphered the molecular mechanism of gall formation with an in vitro infection assay system using RKN Meloidogyne incognita, and the model plant Arabidopsis thaliana. By taking advantages of this system, we performed next-generation sequencing (NGS)-based transcriptome profiling to know molecular mechanisms of nematode infection mechanisms, and found many categories of gene signaling are responsible for nematode infection steps.

Research Products

• [Int'l Joint Research] UNIVERSIDAD DE CASTILLA LA MANCHA(スペイン)
• [Int'l Joint Research] ゲント大学(ベルギー)
• [Int'l Joint Research] INRA(フランス)
• [Int'l Joint Research] ケンブリッジ大学(英国)
• [Int'l Joint Research] UBC(カナダ)
• [Journal Article] CLE peptides and their signaling pathways in plant development. (2016)
  • Author(s): Yamaguchi, Y., Ishida, T.,Sawa, S.
  • Journal Title: J. Exp. Bot. Volume: 67 Issue: 16 Pages: 4813-4826
  • DOI: 10.1093/jxb/erw208
  • Peer Reviewed / Open Access
• [Journal Article] Three-dimensional imaging of plant organs using a simple and rapid transparency technique. (2016)
  • Author(s): Hasegawa J, Sakamoto Y, Nakagami S, Aida M, Sawa S, Matsunaga S
  • Journal Title: Plant Cell Physiol Volume: 57 Issue: 3 Pages: 462-472
  • DOI: 10.1093/pcp/pcw027
  • NAID: 120005756556
  • Peer Reviewed / Open Access
• [Journal Article] The Naming of Names: Guidelines for Gene Nomenclature in Marchantia (2016)
  • Author(s): Bowman, J., Araki, T., Arteaga-Vazquez, M., Berger, F., Dolan, L., Haseloff, J., Ishizaki, K., Kyozuka, J., Lin, S.S., Nagasaki, H., Nakagami, H., Nakajima, K., Nakamura, Y., Ohashi-Ito, K., Sawa, S., Shimamura, M., Solano, R., Tsukaya, H., Ueda, T., Watanabe, Y., Yamato, K.T., Zachgo, S., and Kohchi, T.
  • Journal Title: Plnat cell Physiology Volume: 57 Issue: 2 Pages: 257-261
  • DOI: 10.1093/pcp/pcv193
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Chemotaxis assay of plant-parasitic nematodes on a gel-filled microchannel device (2015)
  • Author(s): Hida, H., Nishiyama, H., Sawa, S., Higashiyama, T., Arata, H.
  • Journal Title: Sensors and Actuators Volume: B 221 Pages: 1483-1491
  • DOI: 10.1016/j.snb.2015.07.081
  • Peer Reviewed
• [Journal Article] BAM 1 and RECEPTOR-LIKE PROTEIN KINASE 2 constitute a signaling pathway and modulate CLE peptide-triggered growth inhibition in Arabidopsis root. (2015)
  • Author(s): Shimizu N, Ishida T, Yamada M, Shigenobu S, Tabata R, Kinoshita A, Yamaguchi K, Hasebe M, Mitsumasu K, Sawa S,
  • Journal Title: New phytologist Volume: 208 Issue: 4 Pages: 1104-1113
  • DOI: 10.1111/nph.13520
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 2.Plant peptide hormone signaling. (2015)
  • Author(s): Motomitsu A, Sawa S, Ishida T
  • Journal Title: Essays in Biochemistry Volume: 58 Pages: 115-131
  • DOI: 10.1042/bse0580115
  • Peer Reviewed
• [Journal Article] Molecular mechanisms of parasitism of plant-parasitic nematodes at apoplast. (2015)
  • Author(s): Mitsumasu, K., Nishiyama, H., Nakagami, S., Kanemaru, Y. and Sawa, S
  • Journal Title: Jap. J. Pesticide Sci Volume: 40 Pages: 44-51
  • NAID: 130005096498
  • Peer Reviewed
• [Journal Article] Mystery in genetics: PUB4 gives a clue to the complex mechanism of CLV signaling pathway in the shoot apical meristem (2015)
  • Author(s): Kinoshita, A., Seo, M., Kamiya, Y., and Sawa, S.
  • Journal Title: Plant Signaling & Behavior Volume: 10:6 Issue: 6 Pages: e1028707-e1028707
  • DOI: 10.1080/15592324.2015.1028707
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Protocol for root-knot nematode culture by a hydroponic system and nematode inoculation to <i>Arabidopsi</i><i>s </i> (2015)
  • Author(s): Nishiyama, H., Ngan, B. T., Nagakami, S., Ejima, C., Ishida, T., and Sawa, S.
  • Journal Title: Nematological Research (Japanese Journal of Nematology) Volume: 45 Issue: 1 Pages: 45-49
  • DOI: 10.3725/jjn.45.45
  • NAID: 130005118607
  • ISSN: 0919-6765, 1882-3408
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A plant U-box protein, PUB4, regulates asymmetric cell division and cell proliferation in the root meristem. (2015)
  • Author(s): Kinoshita, A, Hove, C.A., Tabata, R., Yamada, M., Shimizu, N., Ishida, T., Yamaguchi K., Shigenobu, S., Takebayashi, Y., Iuchi, S., Kobayashi, M., Kurata, T., Wada, T., Seo, M., Hasebe, M., Bilou, I., Fukuda, H., Scheres, B., Heidstra, R., Sawa, S.
  • Journal Title: Development Volume: 142 Issue: 3 Pages: 444-453
  • DOI: 10.1242/dev.113167
  • Peer Reviewed / Open Access
• [Journal Article] Protocol of root-knot nematode culture by hydroponic system and nematode inoculation to Arabidopsis (2015)
  • Author(s): Nishiyama, H., Ngan, B. T., Nagakami, S., Ejima, C., Ishida, T., and Sawa, S
  • Journal Title: Nematological Res. Volume: In press
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Light-dependent green gall formation induced by Meloidogyne incognita. (2014)
  • Author(s): Nishiyama, H., Nakagami, S., Todaka, A., Arita, T., Ishida, T., Sawa, S.
  • Journal Title: Nematology Volume: 16 Issue: 8 Pages: 889-893
  • DOI: 10.1163/15685411-00002814
  • Peer Reviewed
• [Presentation] 植物感染性線虫の感染過程における細胞壁成分の機能と役割 (2017)
  • Author(s): 大田守浩，Allen Yi-Lun Tsai，鈴木れいら，島岡知恵，石田喬志，澤進一郎
  • Organizer: 第58回植物生理学会年会
  • Place of Presentation: 鹿児島大学
  • Year and Date: 2017-03-16
  • Invited
• [Presentation] Analysis of Arabidopsis attractant of plant parasitic nematode, M. incognita. (2016)
  • Author(s): Shinichiro Sawa
  • Organizer: 第57回日本植物生理学会
  • Place of Presentation: 岩手大学上田キャンパス
  • Year and Date: 2016-03-18
  • Invited
• [Presentation] “TOMEI”, a simple and quick clearing method for plant tissues (2015)
  • Author(s): Satoru Nakagami 3, Yuki Katsuyama 2, Shinichiro Sawa 3, Sachihiro Matsunaga
  • Organizer: International ERATO Higashiyama Live-Holonics Symposium and Technical Workshop 2015
  • Place of Presentation: 名古屋大学野依記念学術交流館
  • Year and Date: 2015-08-27
  • Int'l Joint Research
• [Presentation] Analysis of plant attracta nt of plant parasitic nematode, M. incognita (2015)
  • Author(s): Shinichiro Sawa，Satoru Nakagami, Hidetaka Nishiyama, Yumi Kanemaru, Ayane Motomitsu
  • Organizer: Front Lines of Plant Cell Wall Research
  • Place of Presentation: 東大寺総合文化センター
  • Year and Date: 2015-03-20 – 2015-03-21
  • Invited
• [Presentation] Molecular dissection of the CLE signaling pathway on the infection steps of the M. incognita. (2014)
  • Author(s): Shinichrio Sawa, Ayane motomitsu, Hidetaka Nishiyama, Tomomi Sagara, Yumi Kanemaru, Satoru Nakagami, and Takashi Ishida
  • Organizer: 日韓合同線虫学シンポジウム
  • Place of Presentation: 文部科学省研究交流センター
  • Year and Date: 2014-09-18 – 2014-09-19
• [Book] 植物ゲノム解析、次世代シーケンサー活用術 (2016)
  • Author(s): 重信秀治、澤進一郎、栗原志、持田恵一、上原（山口）由紀子、高木宏樹、阿部陽、寺内良平、関原明、高橋聡史
  • Publisher: 化学同人
• [Book] 細胞外空間（細胞壁）に注目した植物寄生性線虫の感染分子機構 (2016)
  • Author(s): 光増可奈子，西山英孝，中上知，金丸由実，澤進一郎
  • Publisher: 日本農薬学会誌
• [Book] Analysis of attractant of plant parasitic nematode (2016)
  • Author(s): Sawa, S
  • Publisher: Soy Protein Research
• [Remarks] The Sawa lab.
  • URL: http://www.sci.kumamoto-u.ac.jp/~sawa/