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The molecular mechanism of uniparental inheraitance of mtDNA analyzed by combining the optical tweezers and microfluidic device.

Research Project

Project/Area Number 26650111
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Morphology/Structure
Research InstitutionKyoto University

Principal Investigator

Nishimura Yoshiki  京都大学, 理学研究科, 助教 (70444099)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords光ピンセット / マイクロ流路 / ミトコンドリア / 片親遺伝 / 遺伝学 / ミトコンドリアDNA / 葉緑体DNA / mtDNA / 母性遺伝 / クリプトコッカス
Outline of Final Research Achievements

Living organisms consist of diverse tissues, cells and organelles, which can be compared to a complex society with various groups and individuals. To decipher the characteristics of individual cells and connections between them, it would be necessary to establish technologies to isolate individual cells for further molecular analyses. For this, we aimed to combine the optical tweezers and microfluidic devises. With this technique, the molecular mechanism of uniparental inheritance of mtDNA was analyzed using Cryptococcus neoformans as a model system, which revealed the active elimination of α-mtDNA in the early developing zygotes that lead to the uniparental inheritance of mtDNA.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (31 results)

All 2017 2016 2015 2014 Other

All Int'l Joint Research (1 results) Journal Article (8 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 8 results,  Open Access: 8 results,  Acknowledgement Compliant: 4 results) Presentation (19 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Book (1 results) Remarks (2 results)

  • [Int'l Joint Research] ブリティッシュコロンビア大学(カナダ)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Dynamic interplay between nucleoid segregation and genome integrity in Chlamydomonas chloroplasts2016

    • Author(s)
      Odahara M, Kobayashi Y (equal contribution), Shikanai T, and Nishimura Y.
    • Journal Title

      Plant Physiology

      Volume: 172 Issue: 4 Pages: 2337-2346

    • DOI

      10.1104/pp.16.01533

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] C-Terminal Region of Sulfite Reductase Is Important to Localize to Chloroplast Nucleoids in Land Plants.2016

    • Author(s)
      Kobayashi, Y., Otani, T., Ishibashi, K., Shikanai, T., *Nishimura, Y.
    • Journal Title

      Genome Biol. Evol.

      Volume: 22 Issue: 5 Pages: 1459-1466

    • DOI

      10.1093/gbe/evw093

    • NAID

      120006822490

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Sequence of the Gonium pectorale Mating Locus Reveals a Complex and Dynamic History of Changes in Volvocine Algal Mating Haplotypes.2016

    • Author(s)
      Hamaji T, Mogi Y, Ferris PJ, Mori T, Miyagishima S, Kabeya Y, Nishimura Y, Toyoda A, Noguchi H, Fujiyama A, Olson BJ, Marriage TN, Nishii I, Umen JG, Nozaki H.
    • Journal Title

      G3

      Volume: 未定 Issue: 5 Pages: 1179-1189

    • DOI

      10.1534/g3.115.026229

    • NAID

      120005997691

    • Related Report
      2016 Annual Research Report 2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] A Cyan Fluorescent Reporter Expressed from the Chloroplast Genome of Marchantia polymorpha.2016

    • Author(s)
      Boehm CR, Ueda M, Nishimura Y, Shikanai T, Haseloff J
    • Journal Title

      Plant Cell Physiol.

      Volume: 57 Issue: 2 Pages: 291-299

    • DOI

      10.1093/pcp/pcv160

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Eukaryotic Components Remodeled Chloroplast Nucleoid Organization during the Green Plant Evolution.2015

    • Author(s)
      Kobayashi Y, Takusagawa M, Harada N, Fukao Y, Yamaoka S, Kohchi T, Hori K, Ohta H, Shikanai T, Nishimura Y.
    • Journal Title

      Genome Biol Evol.

      Volume: 8 Issue: 1 Pages: 1-16

    • DOI

      10.1093/gbe/evv233

    • NAID

      120006822487

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] RECA plays a dual role in the maintenance of chloroplast genome stability in Physcomitrella patens.2015

    • Author(s)
      Odahara, M., Inouye, T., Nishimura, Y., Sekine, Y.
    • Journal Title

      Plant J.

      Volume: 84 Issue: 3 Pages: 516-526

    • DOI

      10.1111/tpj.13017

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Development of Gateway binary vector series with four different selection markers for the liverwort Marchantia polymorpha.2015

    • Author(s)
      Ishizaki, K., Nishihama, R., Ueda, M., Inoue, K., Ishida, S., Nishimura., Y., Shikanai, T., Kohchi, T
    • Journal Title

      PLoS ONE

      Volume: 10 Issue: 9 Pages: e0138876-e0138876

    • DOI

      10.1371/journal.pone.0138876

    • NAID

      120005715313

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Algae sense exact temperatures: small heat shock proteins are expressed at the survival threshold temperature in Cyanidioschyzon merolae and Chlamydomonas reinhardtii.2014

    • Author(s)
      Kobayashi Y, Harada N, Nishimura Y, Saito T, Nakamura M, Fujiwara T, Kuroiwa T, Misumi O.
    • Journal Title

      Genome Biol Evol.

      Volume: 6 Issue: 10 Pages: 2731-2740

    • DOI

      10.1093/gbe/evu216

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Identification of a Holliday junction resolvase in chloroplasts2017

    • Author(s)
      Kobayashi, Y., Misumi, O., Odahara, M., Hirono, M., Hidaka, K., Endo, M., Sugiyama, H., Kuroiwa, T., Shikanai, T., Nishimura, Y.
    • Organizer
      第58回日本植物生理学会年会
    • Place of Presentation
      鹿児島大学、鹿児島
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] Dynamics of chloroplast nucleoid divisions captured by microfluidic device2017

    • Author(s)
      Kamimura, Y., Kobayashi, Y., Shikanai, T., Nishimura, Y.
    • Organizer
      第58回日本植物生理学会年会
    • Place of Presentation
      鹿児島大学、鹿児島
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] Mechanism for the cell-type specific expression of the chloroplast ndh genes in Zea mays.2017

    • Author(s)
      Yano, H., Ishibashi, K., Nishimura, Y., Shikanai, T.
    • Organizer
      第58回日本植物生理学会年会
    • Place of Presentation
      鹿児島大学、鹿児島
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] Visualization of chloroplast-targeted autophagy in Chlamydomonas reinhardtii2017

    • Author(s)
      Li, N., Nishimura, Y., Ramundo, S., Hidema, J., Izumi, M.
    • Organizer
      第58回日本植物生理学会年会
    • Place of Presentation
      鹿児島大学、鹿児島
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] オルガネラ核様体のダイナミズム2017

    • Author(s)
      西村芳樹
    • Organizer
      京都産業大学 バイオフォーラム
    • Place of Presentation
      京都産業大学、京都
    • Year and Date
      2017-02-16
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 亜硫酸還元酵素のC 末端領域は葉緑体核様体局在に重要である2016

    • Author(s)
      小林優介, 鹿内利治, 西村芳樹
    • Organizer
      日本植物学会第80回大会
    • Place of Presentation
      沖縄コンベンションセンター、沖縄
    • Year and Date
      2016-09-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] Restructuring of chloroplast nucleoid factors during the green plant evolution2016

    • Author(s)
      Nishimura, Y.
    • Organizer
      The 13th international colloquium of Endocytobiology and Symbiosis
    • Place of Presentation
      京都府立大学、京都
    • Year and Date
      2016-09-10
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Active degradation of mitochondrial nucleoid as the driving force for uniparental inheritance in Cryptococcus.2016

    • Author(s)
      Yoshiki Nishimura,Mari Takusagawa, Toshiharu Shikanai,Akio Toh-e
    • Organizer
      日本植物学会第77回大会
    • Place of Presentation
      岩手大学
    • Year and Date
      2016-03-18
    • Related Report
      2015 Research-status Report
  • [Presentation] Analysis of regulatory mechanism of cell-type specific expression of chloroplast ndh genes in Zea mays2016

    • Author(s)
      Haruna Yano, Yuya Fukuta, Kota Ishibashi, Yoshiki Nishimura, Toshiharu Shikanai
    • Organizer
      日本植物学会第77回大会
    • Place of Presentation
      岩手大学
    • Year and Date
      2016-03-18
    • Related Report
      2015 Research-status Report
  • [Presentation] Genome instability in the Chlamydomonas chloroplasts impaired in nucleoid segregation2016

    • Author(s)
      Masaki Odahara, Yusuke Kobayashi, Osami Misumi, and Yoshiki Nishimura
    • Organizer
      日本植物学会第77回大会
    • Place of Presentation
      岩手大学
    • Year and Date
      2016-03-18
    • Related Report
      2015 Research-status Report
  • [Presentation] Remodeling of chloroplast nucleoid organization by eukaryotic components during the green plant evolution2016

    • Author(s)
      Yusuke Kobayashi, Mari Takusagawa, Naomi Harada, Yoichiro Fukao, Shohei Yamaoka, Takayuki Kohchi, Koichi Hori, Hiroyuki Ohta, Toshiharu Shikanai, Yoshiki Nishimura
    • Organizer
      日本植物学会第77回大会
    • Place of Presentation
      岩手大学
    • Year and Date
      2016-03-18
    • Related Report
      2015 Research-status Report
  • [Presentation] 葉緑体核様体コア因子の多様性と進化2015

    • Author(s)
      小林優介、田草川真理、原田尚実、深尾陽一朗、山岡尚平、河内孝之、堀孝一、太田啓之、
    • Organizer
      日本植物学会第79回大会
    • Place of Presentation
      朱鷺メッセ、新潟
    • Year and Date
      2015-09-06
    • Related Report
      2015 Research-status Report
  • [Presentation] ミトコンドリア核様体構造からみた母性遺伝の分子機構2015

    • Author(s)
      西村芳樹、田草川真理、鹿内利治、東江昭夫
    • Organizer
      日本植物学会第79回大会
    • Place of Presentation
      朱鷺メッセ、新潟
    • Year and Date
      2015-09-06
    • Related Report
      2015 Research-status Report
  • [Presentation] 葉緑体核様体構造の大進化2015

    • Author(s)
      小林優介、田草川真理、原田尚実、深尾陽一朗、山岡尚平、河内孝之、堀孝一、太田啓之、
    • Organizer
      日本植物形態学会第26回大会
    • Place of Presentation
      朱鷺メッセ、新潟
    • Year and Date
      2015-09-05
    • Related Report
      2015 Research-status Report
  • [Presentation] ミトコンドリア核様体は片親遺伝における制御因子である2015

    • Author(s)
      西村芳樹、 田草川真理、 鹿内利治、 東江昭夫
    • Organizer
      日本植物形態学会第26回大会
    • Place of Presentation
      朱鷺メッセ、新潟
    • Year and Date
      2015-09-05
    • Related Report
      2015 Research-status Report
  • [Presentation] 緑藻クラミドモナスからみえて来た葉緑体核様体の形態制御機構2015

    • Author(s)
      上村嘉誉、小林優介、鹿内利治、西村芳樹
    • Organizer
      クラミドモナスワークショップ
    • Place of Presentation
      中央大学、東京
    • Year and Date
      2015-09-03
    • Related Report
      2015 Research-status Report
  • [Presentation] ミトコンドリア片親遺伝をつかさどる複層的分子機構2015

    • Author(s)
      西村芳樹、鹿内利治、東江昭夫
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大学 世田谷キャンパス、東京
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Research-status Report
  • [Presentation] 母性遺伝を引き起こす片親ミトコンドリアの選択的排除機構2014

    • Author(s)
      西村芳樹、鹿内利治、東江昭夫
    • Organizer
      日本植物学会第78回大会
    • Place of Presentation
      明治大学生田キャンパス、神奈川
    • Year and Date
      2014-09-12 – 2014-09-14
    • Related Report
      2014 Research-status Report
  • [Presentation] Deciphering the mechanisms underlying the uniparental inheritance of chloroplast and mitochondrial DNA2014

    • Author(s)
      Nishimura, Y.
    • Organizer
      Gordon Research Conference, Mitochondria and Chloroplast
    • Place of Presentation
      Lucca (Barga), Italy
    • Year and Date
      2014-07-06 – 2014-07-11
    • Related Report
      2014 Research-status Report
  • [Book] Active digestion of paternal chloroplast DNA in a young zygote of Chlamydomonas reinhardtii: the basis for maternal inheritance. Atlas in Plant Cell Structure, Chapter 32014

    • Author(s)
      Nishimura, Y.
    • Total Pages
      202
    • Publisher
      Springer
    • Related Report
      2014 Research-status Report
  • [Remarks] 京都大学大学院 理学研究科 植物分子遺伝学研究室

    • URL

      http://www.bot.kyoto-u.ac.jp/j/5_iden.html

    • Related Report
      2015 Research-status Report
  • [Remarks] 植物学教室ー植物分子遺伝学

    • URL

      http://www.bot.kyoto-u.ac.jp/j/5_iden.html

    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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