Experimental verification of gene loss patterns in yeast genome after whole genome duplication

• Project/Area Number: 26650130
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Evolutionary biology
• Research Institution: Tohoku University
• Principal Investigator: Makino Takashi 東北大学, 生命科学研究科, 准教授 (20443442)
• Co-Investigator(Kenkyū-buntansha): Moriya Hisao 岡山大学, 異分野融合先端研究コア, 准教授 (60500808)
• Co-Investigator(Renkei-kenkyūsha): Kawata Masakado 東北大学, 大学院生命科学研究科, 教授 (90204734)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 全ゲノム重複 / 進化実験 / 酵母 / ゲノム重複 / 比較ゲノム

Outline of Final Research Achievements

The purpose of this study is to establish an artificial evolutionary experiment approach for tetraploid yeast, and examine the pattern of gene function losses. We cultured tetraploid yeast for 200 days under exposure to intense ultraviolet irradiation. As a result, we observed many mutations in the genome of tetraploid yeast. In particular, deleterious mutations were enriched in genes on the same chromosome of functional gene clusters.

Research Products

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  • URL: http://meme.biology.tohoku.ac.jp/klabo-wiki/index.php?%CB%D2%CC%EE%C7%BD%BB%CE