Analysis of male gametes during pollen tube growth by single cell omics with in vitro pollen culture system

Research Project

Project/Area Number	26660017
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Horticultural science
Research Institution	Hokkaido University
Principal Investigator	HOSHINO Yoichiro 北海道大学, 北方生物圏フィールド科学センター, 准教授 (50301875)
Project Period (FY)	2014-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000) Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000) Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords	花粉 / 花粉管 / 花粉管伸長 / オミクス
Outline of Final Research Achievements	Alstroemeria, Petunia and Paeonia, which have bicellular pollen, were used for analyzing pollen tube development and nuclear phase change in liquid pollen culture medium. The pollen grains of these plant species could germinate in vitro and produce sperm cells through mitosis of generative cell. Proteome analysis was applied to pollen grain and in vitro grown-pollen tubes in Alstroemeria. During pollen tube growth, some proteins were identified with mass spectrometry.

Report

(3 results)