Inositol synthesis in Sinorhizobium meliloti involved in salt tolerance
| Project/Area Number |
26660067
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | Kobe University |
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Principal Investigator |
Yoshida Ken-ichi 神戸大学, (連合)農学研究科(研究院), 教授 (20230732)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | Sinorhizobium meliloti / inositol / salt stress / osmotic stress / myo-inositol / 国際情報交換 |
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| Outline of Final Research Achievements |
Sinorhizobium meliloti possesses ino1 encoding myo- inositol phosphate synthase, which is induced upon salt stress, implying the possibility that inositol derivatives might function in this bacterium as an osmoprotectant. On the other hand, this bacterium has several other osmoprotectants, including glutamic acid, N- acetyl Guru Tamil glutamine amides, betaines, and trehalose, and the effects of ino1 might not be so obvious. A series of mutant strains with various combinations of inactivated genes indicated that ino1 could affect osmoresistance, but its contribution had only auxiliary significance. Therefore, an attempt was made to examine if overexpressed ino1 might enhance the resistance. However, we failed to overexpress ino1 after multiple trials, and over-expression of ino1 might have some toxicity. In any case, S. meliloti could produce myo-inositol phosphate, which is induced in response to salt stress and involved in salt tolerance acquisition.
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Report
(3 results)
Research Products
(9 results)
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[Journal Article] Hyperphosphorylation of DegU cancels CcpA-dependent catabolite repression of rocG in Bacillus subtilis2015
Author(s)
Tanaka, K., Iwasaki, K., Morimoto, T., Matsuse, T., Hasunuma, T., Takenaka, S., Chumsakul, O., Ishikawa, S., Ogasawara, N., and Yoshida, K.
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Journal Title
BMC Microbiol
Volume: 15
Issue: 1
Pages: 43-43
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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