| Project/Area Number |
26660143
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Wood science
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
鈴木 史朗 京都大学, 生存圏研究所, 助教 (70437268)
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| Co-Investigator(Renkei-kenkyūsha) |
UTSUMI Ryutaro 近畿大学, 農学部, 教授 (20151912)
SHIBATA Daisuke かずさDNA研究所, 産業基盤開発研究部, 部長 (10370925)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | リグニン / リグナン / 芳香核 / 単純化 / デメチル化 / セコイソラリシレジノール / マタイレジノール / 哺乳動物リグナン / 乳がん / 芳香核変換 / 脱メチル化 / 脱水酸化 |
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| Outline of Final Research Achievements |
A human intestinal bacterium Blautia producta ATCC27340, whose draft genomic sequence has been released, demethylated two lignans, secoisolariciresinol and matairesinol. The bacterium demethylated secoisolariciresinol faster than matairesinol, and aromatic B-ring of matairesinol was preferentially demethylated. Analysis of draft genome of the bacterium revealed that the genomic sequence contained a cluster that encoded four proteins required for tetrahydrofolate-dependent demethylation of aromatic methoxyl groups. We obtained the complementary DNAs of the four proteins to set up lignan demethylation assay using the recombinant proteins.
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