• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Identification of the target molecule of Ganoderma triterpenoid

Research Project

Project/Area Number 26660147
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Wood science
Research InstitutionKyushu University

Principal Investigator

shimizu kuniyoshi  九州大学, (連合)農学研究科(研究院), 准教授 (20346836)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords霊芝 / Ganoderma / トリテルペノイド / 標的タンパク質 / チューブリン / ケミカルバイオロジー / ganoderic acid DM / マンネンタケ / 磁性ビーズ / ガノデリン酸 / ガノデルマアルコール / 抗癌活性 / 癌細胞
Outline of Final Research Achievements

The fungus Ganoderma lingzhi (Reishi, Mannentake, or Lingzhi) has been used for centuries in East Asia to treat various human diseases. Up to now, over 120 kinds of triterpenoids have been isolated from the genus Ganoderma. We have investigated anti-androgen effect and the inhibitory effects of osteoclastic differentiation of triterpenoids isolated from this mushroom in vitro. Ganoderic acid DM showed anti-androgenic activity which showed potent 5α-reductase inhibiting effect. Also, ganoderic acid DM inhibited the osteoclastic differentiation, which responding to receptor activator of NF-κB ligand (RANKL) and a tumor necrosis factor α (TNF-α). These results led us to investigate the target molecule of ganoderic acid DM by using affinity bead method. In this study, beta-tublin is identified as one of the target molecule of ganoderic acid DM in LNCaP cells. This result should be very helpful in elucidating the unsolved function of Ganoderma mushroom.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (19 results)

All 2016 2015 2014 Other

All Journal Article (9 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 8 results,  Open Access: 2 results,  Acknowledgement Compliant: 3 results) Presentation (6 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results) Remarks (1 results) Patent(Industrial Property Rights) (3 results)

  • [Journal Article] Lucidumol C, a new lanostanoid triterpene from Ganoderma lingzhi with potential activity against colon cancer2016

    • Author(s)
      Yhiya M. Amen, Amira Mira, Hai-Bang Tran, Mohamed S. Afifi, Ahmed F. Halim, Ahmed Ashour, Kuniyoshi Shimizu
    • Journal Title

      Journal of Natural Medicines

      Volume: in press

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Methoxyflavones from Ganoderma lingzhi2016

    • Author(s)
      Yhiya M. Amen, Satoru Kaifuchi, Kuniyoshi Shimizu
    • Journal Title

      International Journal of Medicinal Mushrooms

      Volume: in press

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Detection of ganoderic acid A in Ganoderma lingzhi by an indirect competitive enzyme-linked immunosorbent assay2016

    • Author(s)
      Sakamoto, S., Kohno, T., Shimizu, K., Tanaka, H., Morimoto, S.
    • Journal Title

      Planta Med.

      Volume: in press

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 霊芝の多機能性薬理効果に関する近年の科学的検証2016

    • Author(s)
      清水 邦義、朱 欽昌、吉村 友里、海渕 覚、桑原 李佳、大貫 宏一郞
    • Journal Title

      FFIジャーナル

      Volume: 印刷中

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Inhibition of neuraminidase by Ganoderma triterpenoids and implications for neuraminidase inhibitor design.2015

    • Author(s)
      Zhu Q, Bang TH, Ohnuki K, Sawai T, Sawai K, Shimizu K.
    • Journal Title

      Sci Rep.

      Volume: 5 Issue: 1 Pages: 13194-13202

    • DOI

      10.1038/srep13194

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structure-Activity Relationship and Inhibition Pattern of Reishi-derived (Ganoderma lingzhi) Triterpenoids against Angiotensin-Converting Enzyme2015

    • Author(s)
      T. H. Bang, K. Shimizu
    • Journal Title

      Phytochemistry Letters

      Volume: in press

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Hypotensive effects and angiotensin-converting enzyme inhibitory peptides of reishi (Ganoderma lingzhi) auto-digested extract2014

    • Author(s)
      T. H. Bang, A. Yamamoto, S. Matsumoto, H. Ito, K. Igami, T. Miyazaki, R. Kondo, K. Shimizu
    • Journal Title

      Molecules

      Volume: 19 Issue: 9 Pages: 13473-13485

    • DOI

      10.3390/molecules190913473

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Potent Angiotensin-Converting Enzyme Inhibitory Tripeptides identified by a Computer-based Approach2014

    • Author(s)
      T. H. Bang, K. Shimizu
    • Journal Title

      Journal of Molecular Graphics and Modeling

      Volume: 53 Pages: 206-211

    • DOI

      10.1016/j.jmgm.2014.08.002

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] 多機能性キノコ「霊芝」(Ganoderma lingzhi)の更年期症状改善効果2014

    • Author(s)
      清水邦義、河野俊亨
    • Journal Title

      更年期と加齢のヘルスケア

      Volume: 13 Pages: 1-7

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] The Multi-functional effects of triterpenpoids isolated from Ganoderma lingzhi2016

    • Author(s)
      Kuniyoshi SHIMIZU(Invitred lecture)
    • Organizer
      The 55th Aniversary of National Institute of Medicinal Materials (NIMM) conference
    • Place of Presentation
      La Thanh Hotel, 226 Van Phuc Street, Ba Dinh District, Hanoi
    • Year and Date
      2016-04-13
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Inhibition Effect of Ganoderma Triterpenoids against Influenza Neuraminidase and Implications for Neuraminidase Inhibitor Design2015

    • Author(s)
      Qinchang ZHU, Tran Hai BANG, Koichiro OHNUKI, Takashi SAWAI, Ken SAWAI,Kuniyoshi SHIMIZU
    • Organizer
      第38回日本分子生物学会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2015-12-01 – 2015-12-04
    • Related Report
      2014 Research-status Report
  • [Presentation] Multi-functional effects of triterpenoids isolated from Ganoderma mushroom2015

    • Author(s)
      Kuniyoshi SHIMIZU (Invited Keynote lecture)
    • Organizer
      The First Chinese Lingzhi Conference
    • Place of Presentation
      Longquan, China
    • Year and Date
      2015-08-09
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Development of an indirect competitive enzyme-linked immunosorbent assay (icELISA) using highly specific monoclonal antibody against ganoderic acid A2015

    • Author(s)
      Toshitaka Kohno, Kuniyoshi Shimizu, Seiichi Sakamoto, Hiroyuki Tanaka, Satoshi Morimoto
    • Organizer
      The international conference on herbal and traditional medicine
    • Place of Presentation
      Khon Kaen University, Thailand
    • Year and Date
      2015-01-28 – 2015-01-30
    • Related Report
      2014 Research-status Report
  • [Presentation] Tubulin polymerizing activity of Ganoderma triterpenoids and production of their monoclonal antibody2014

    • Author(s)
      Toshitaka Kohno, Tran Hai Bang, Kuniyoshi Shimizu, Seiichi Sakamoto, Hiroyuki Tanaka, Satoshi Morimoto
    • Organizer
      The 8th JSP-CCTCNM-KSP Joint Symposium on Pharmacognosy
    • Place of Presentation
      福岡大学
    • Year and Date
      2014-09-13
    • Related Report
      2014 Research-status Report
  • [Presentation] Inhibition of angiotensin converting enzyme by lanostanoids from reishi (Ganoderma lingzhi): structure-activity relationship and inhibition pattern2014

    • Author(s)
      Hai Bang TRAN, Kuniyoshi SHIMIZU
    • Organizer
      The 8th JSP-CCTCNM-KSP Joint Symposium on Pharmacognosy
    • Place of Presentation
      福岡大学
    • Year and Date
      2014-09-13
    • Related Report
      2014 Research-status Report
  • [Remarks] 研究業績

    • URL

      http://ffpsc.agr.kyushu-u.ac.jp/sffps/shimizu_result.html#genchoronbun

    • Related Report
      2015 Annual Research Report
  • [Patent(Industrial Property Rights)] ノイラミニダーゼ阻害剤、これを含有した抗インフルエンザ剤、食品および薬剤、並びにそれらの製造方法2014

    • Inventor(s)
      清水邦義、澤井健
    • Industrial Property Rights Holder
      九州大学、東洋丹芝園
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2014-170877
    • Filing Date
      2014-08-25
    • Related Report
      2014 Research-status Report
  • [Patent(Industrial Property Rights)] アンギオテンシン変換酵素(ACE)の阻害剤、これを含む食品および薬剤、並びにそれらの製造方法2014

    • Inventor(s)
      清水邦義、澤井健
    • Industrial Property Rights Holder
      九州大学、東洋丹芝園
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2014-170876
    • Filing Date
      2014-08-25
    • Related Report
      2014 Research-status Report
  • [Patent(Industrial Property Rights)] チューブリン重合活性調節剤、これを含む食品および薬剤、並びにチューブリン重合活性調節剤の製造方法2014

    • Inventor(s)
      清水邦義、澤井健
    • Industrial Property Rights Holder
      九州大学、東洋丹芝園
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2014-135844
    • Filing Date
      2014-07-01
    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2017-05-10  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi