Kinship-based population genetic study for the future stock enhancement program of Japanese flounder

• Project/Area Number: 26660157
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Aquatic bioproduction science
• Research Institution: Tohoku University
• Principal Investigator: Ikeda Minoru 東北大学, (連合)農学研究科(研究院), 准教授 (70232204)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: ヒラメ / 栽培漁業 / 人工種苗 / 遺伝的多様性 / マイクロサテライトDNA / ミトコンドリアDNA / 親子鑑定 / 血縁鑑定 / 放流効果 / ゲノミクス / 血縁関係

Outline of Final Research Achievements

Sibship reconstruction using DNA markers is useful for monitoring the reproductive success of the captive broodstock in hatchery and the offspring stocked into the wild. We prepared highly polymorphic DNA marker sets of 12 microsatellites (ms) DNA and 1,873 nucleotides in mitochondrial (mt) DNA of Japanese flounder. Although the footprint of natural selection in the wild populations could not be detected by genome scanning methods, these marker sets were extremely helpful to reconstruct sibship in the artificial and wild populations without the parental information. Negative genetic effects to wild population, such as decreasing genetic diversity, promoted by improper stock enhancement program should be detected as increasing of closely related individuals within populations. We preliminary simulated the genetic effect to wild population after the stocking of artificial seeds produced from a captive wild broodstock using the sibship reconstruction system.

Research Products

• [Presentation] An impact of stocking of wild population with Japanese flounder Paralichthys olivaceus on “stocking and reproductive simulation” (2015)
  • Author(s): Ando D., Ikeda M., Sugaya T., Katamachi D., Fujii T., Kijima A.
  • Organizer: The 5th International Symposium on Stock Enhancement and Sea Ranching
  • Place of Presentation: シドニー（オーストラリア）
  • Year and Date: 2015-10-11
  • Int'l Joint Research
• [Presentation] Development of the system to determine parantage and sibship in hatchery population for stock enhancement program of Japanese flounder Paralichthys olivaceus (2015)
  • Author(s): Ando D., Ikeda M., Sugaya T., Katamachi D., Fujii T., Kijima A.
  • Organizer: ISGA XII – The international symposium on genetics in aquaculture XII
  • Place of Presentation: サンチャゴ コンポステラ（スペイン）
  • Year and Date: 2015-06-21
  • Int'l Joint Research