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Understanding of the ecological characteristics and genetic variation of symbiotic zooxanthellae within giant clam for stable seed production of giant clam species

Research Project

Project/Area Number 26660164
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Aquatic bioproduction science
Research InstitutionFisheries Research and Education Agency

Principal Investigator

Yamashita Hiroshi  国立研究開発法人水産研究・教育機構, 西海区水産研究所, 研究員 (00583147)

Co-Investigator(Kenkyū-buntansha) 小池 一彦  広島大学, 生物圏科学研究科, 教授 (30265722)
Research Collaborator Kondo Shi-nobu  沖縄県水産海洋技術センター
Minami Yoichi  沖縄県水産海洋技術センター
Inoue Ken  沖縄県水産海洋技術センター
Ikeda Shota  広島大学
Osada Hiroki  広島大学
Morishima Shin-ya  広島大学
Mukai Kazuma  広島大学
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords増養殖 / 水産学 / 生態学 / 共生生物学 / 生物圏現象 / 褐虫藻 / シャコガイ / 種苗生産
Outline of Final Research Achievements

We investigated Symbiodinium clade compositions within two giant clam species, Tridacna crocea and Tridacna squamosa, maintained in the same outdoor pond. Most of T. crocea harbored only clade A Symbiodinium, whereas multiple clades were detected within most of T. squamosa. In the case of T. squamosa, along with increasing the clam size, the main dominant clade shift clade A to C, and decreases in Symbiodinium genetic diversity. In the laboratory experiment using T. squamosa larvae, there is an increased mortality rate of the larvae when stopped Symbiodinium cell supply. We then introduced these information to seed production of T. squamosa. Namely, Symbiodinium that is considered to have high genetic diversity squeezed out from small T. squamosa was supplied constantly to T. squamosa larvae. The symbiosis rate of this method was about two times higher than that of traditional method.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (6 results)

All 2017 2016 2014 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results,  Acknowledgement Compliant: 2 results) Presentation (3 results) Remarks (1 results)

  • [Journal Article] Zooxanthellal genetic varieties in giant clams are partially determined by species-intrinsic and growth-related characteristics2017

    • Author(s)
      Shota Ikeda, Hiroshi Yamashita, Shi-nobu Kondo, Ken Inoue, Shin-ya Morishima, Kazuhiko Koike
    • Journal Title

      PLoS ONE

      Volume: 12(2) Issue: 2 Pages: e0172285-e0172285

    • DOI

      10.1371/journal.pone.0172285

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] A simple and rapid determination method for zooxanthellal genetic diversity in giant clams using multiplex PCR2016

    • Author(s)
      Shota Ikeda, Hiroshi Yamashita, Lawrence M. Liao, Kazuhiko Koike
    • Journal Title

      Fisheries Science

      Volume: 82 Issue: 5 Pages: 747-753

    • DOI

      10.1007/s12562-016-1004-x

    • NAID

      40020941515

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 効率的なヒレジャコ種苗生産のための褐虫藻添加手法2017

    • Author(s)
      山下洋,南洋一,近藤忍,井上顕,小池一彦
    • Organizer
      平成29年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(品川キャンパス)
    • Related Report
      2016 Annual Research Report
  • [Presentation] シャコガイの「種」と「成長」が,共生する褐虫藻の遺伝的多様性を左右する2016

    • Author(s)
      森島慎也,向井春摩,池田正太,小池一彦,近藤 忍,南 洋一,山下 洋
    • Organizer
      平成28年度 日本水産学会春季大会
    • Place of Presentation
      東京海洋大学品川キャンパス
    • Year and Date
      2016-03-28
    • Related Report
      2015 Research-status Report
  • [Presentation] シャコガイに共生する褐虫藻の多様性2014

    • Author(s)
      池田正太、山下 洋、近藤忍、小池一彦
    • Organizer
      第17回日本サンゴ礁学会
    • Place of Presentation
      高知県高知市
    • Year and Date
      2014-11-27 – 2014-11-30
    • Related Report
      2014 Research-status Report
  • [Remarks] 西海区水産研究所主要研究成果集 第21号 (平成28年度)

    • URL

      http://snf.fra.affrc.go.jp/seika/index.html

    • Related Report
      2016 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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