Diagnostic halmarks and pathogenesis of canine inflammatory bowel disease and T cell lymphomas.

• Project/Area Number: 26660235
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Veterinary medical science
• Research Institution: The University of Tokyo
• Principal Investigator: Uchida kazuyuki 東京大学, 農学生命科学研究科, 准教授 (10223554)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 犬 / 炎症性腸疾患 / T細胞性リンパ腫 / セリアック病 / グリアジン / 組織トランスグルタミナーゼ / 慢性腸炎 / リンパ腫 / グリアジン抗体 / 組織トランスグルタミナーゼ抗体 / 内視鏡検査

Outline of Final Research Achievements

Molecular clonality analysis of T-cell receptor (TCR) genes for diagnosing T-cell lymphoma is widely used in veterinary medicine. However, differentiating chronic enteritis (CE) from intestinal lymphoma is challenging. We conducted serological examinations in combination IgA and IgG antibodies against gliadin and tissue transglutaminase (tTG) were quantitatively measured using ELISA. The conditions were classified according to the histopathological diagnosis, clonality analysis, and combined histopathological/clonality analysis. Histopathological analysis showed that dogs with intestinal lymphoma were likely to have high levels of serum IgA antibodies against gliadin and tTG, and serum IgG antibodies against tTG. Interestingly, dogs with intestinal lymphoma had a higher serum IgA titer against gliadin and tTG than did dogs with CE. These results suggest that canine CE and intestinal lymphoma may have a pathogenesis similar to human Celiac disease.

Research Products

• [Journal Article] Changes in Foxp3-Positive Regulatory T Cell Number in the Intestine of Dogs With Idiopathic Inflammatory Bowel Disease and Intestinal Lymphoma. (2016)
  • Author(s): Maeda S, Ohno K, Fujiwara-Igarashi A, Uchida K, Tsujimoto H.
  • Journal Title: Veterinary Pathology Volume: 53(1) Issue: 1 Pages: 102-112
  • DOI: 10.1177/0300985815591081
  • Peer Reviewed / Open Access
• [Journal Article] Association between lymphocyte antigen receptor gene rearrangements and histopathological evaluation in canine chronic enteropathy. (2015)
  • Author(s): Hiyoshi S, Ohno K, Uchida K, Goto-Koshino Y, Nakashima K, Fukushima K, Kanemoto H, Maeda S, Tsujimoto H.
  • Journal Title: Vet Immunol Immunopathol. Volume: 165(3-4) Issue: 3-4 Pages: 138-144
  • DOI: 10.1016/j.vetimm.2015.03.009
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Prognostic factors in dogs with protein-losing enteropathy. (2015)
  • Author(s): Nakashima K, Hiyoshi S, Ohno K, Uchida K, Goto-Koshino Y, Maeda S, Mizutani N, Takeuchi A, Tsujimoto H.
  • Journal Title: Vet J Volume: 205(1) Issue: 1 Pages: 28-32
  • DOI: 10.1016/j.tvjl.2015.05.001
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Construction of a multicolor GeneScan analytical system to detect clonal rearrangements of immunoglobulin and T cell receptor genes in canine lymphoid tumors. (2015)
  • Author(s): Goto-Koshino, Y., Mochizuki, H., Sato, M., Nakashima, K., Hiyoshi, S., Fujiwara-Igarashi, A., Maeda, S., Nakamura, K., Uchida, K., Fujino, Y., Ohno, K. and Tsujimoto, H.
  • Journal Title: Veterinary Immunology and Immunopathology Volume: In press Issue: 1-2 Pages: 81-87
  • DOI: 10.1016/j.vetimm.2015.03.005
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 犬の慢性腸炎および腸管リンパ腫の病理発生に関する研究 (2016)
  • Author(s): 松本 功、内田和幸（他4名）
  • Organizer: 第3回JCVP学術集会
  • Place of Presentation: 三鷹市民公会堂、三鷹市、東京都
  • Year and Date: 2016-03-29