| Project/Area Number |
26660254
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Integrative animal science
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| Research Institution | National Agriculture and Food Research Organization |
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Principal Investigator |
EGUCHI Masahiro 国立研究開発法人農業・食品産業技術総合研究機構, 動物衛生研究部門 細菌・寄生虫研究領域, 上級研究員 (00312215)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | サルモネラ / モノクローナル抗体 / 液性免疫応答 / 液性免疫 / F(ab)2 / 感染防御 |
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| Outline of Final Research Achievements |
Salmonella enterica subsp. enterica is one of the most common causative agents of gastroenteritis and systemic infections worldwide. It has been shown that both T and B cells might contribute to protective immunity against this pathogen. While CD4 T cell response is generally recognized as crucial, the role of B cells and antibodies in immunity against Salmonella is less clear and often controversial. Therefore we set off to study interactions between Salmonella Typhimurium and a monoclonal antibody mAb414 raised against one of its major antigens, lipopolysaccharide (LPS).
The potential of the antibody against Salmonella LPS to protect against the infection is mainly attributed to signaling to leukocytes. Nevertheless, our in vitro experiments suggest that anti-LPS antibody might itself trigger changes on the bacterial surface as well as in metabolic pathways. Further studies will have to clarify, whether these effects are caused by a mere binding of antibody to LPS or by agglutination.
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