Expression and storage of genetic information in insect cells

Research Project

Project/Area Number	26660269
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Insect science
Research Institution	Kyushu University
Principal Investigator	Kusakabe Takahiro 九州大学, (連合)農学研究科(研究院), 教授 (30253595)
Project Period (FY)	2014-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000) Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000) Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords	クロマチン構造 / インシュレーター / DNAメチル化 / 昆虫 / 核構造
Outline of Final Research Achievements	High-ordered chromatin structure plays important roles in expression and storage of genetic information. In this study, we analyzed the molecular mechanisms of DNA methylation and its regulation in insect cells. DNA methylation-related genes were cloned from silkworm cells and their biochemical activities are characterized in detail. All together silkworm genes whose expression profiles were affected by DNA methylation were identified. Also the genes for insulator proteins blocking the effect of distant enhancers and spread of heterochromatin formation beyond chromatin loops were identified from silkworm cells and their structures and molecular functions were analyzed.

Report

(3 results)