Expression and storage of genetic information in insect cells
Project/Area Number |
26660269
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Insect science
|
Research Institution | Kyushu University |
Principal Investigator |
Kusakabe Takahiro 九州大学, (連合)農学研究科(研究院), 教授 (30253595)
|
Project Period (FY) |
2014-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | クロマチン構造 / インシュレーター / DNAメチル化 / 昆虫 / 核構造 |
Outline of Final Research Achievements |
High-ordered chromatin structure plays important roles in expression and storage of genetic information. In this study, we analyzed the molecular mechanisms of DNA methylation and its regulation in insect cells. DNA methylation-related genes were cloned from silkworm cells and their biochemical activities are characterized in detail. All together silkworm genes whose expression profiles were affected by DNA methylation were identified. Also the genes for insulator proteins blocking the effect of distant enhancers and spread of heterochromatin formation beyond chromatin loops were identified from silkworm cells and their structures and molecular functions were analyzed.
|
Report
(3 results)
Research Products
(2 results)
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[Journal Article] Biochemical characterization of maintenance DNA methyltransferase DNMT-1 from silkworm, Bombyx mori2015
Author(s)
Mitsudome T, Mon H, Xu J, Li Z, Lee JM, Patil AA, Masuda A, Iiyama K, Morokuma D, Kusakabe T.
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Journal Title
Insect Biochemistry and Molecular Biology
Volume: 58
Pages: 55-65
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Presentation] DNA methyltransferase DNMT-1 from Bombyx mori2015
Author(s)
Masato Hino・Takumi Mitsudome・Hiroaki Mon・Masanao Sato・Yoshitaka Suetsugu・Kimiko Yamamoto・Zhiqing Li・Jian Xu・Li Zhu・JaeMan Lee・Takahiro Kusakabe
Organizer
International Symposium on Basic and Applied Research on Sericulture and Insect Sciences
Place of Presentation
韓国大邱市
Year and Date
2015-04-20
Related Report
Int'l Joint Research