| Project/Area Number |
26660294
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Yamaguchi University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
AKAO Yukihiro 岐阜大学, 大学院連合創薬医療情報研究科, 教授 (00222505)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | エクソソーム / Exosome / microRNA-205 / CD9 / メラノーマ / exosome / TGF-β |
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| Outline of Final Research Achievements |
This study revealed that TGF-β, which promotes the differentiation of T cells to regulatory T cells, was included in the exosomes derived from melanoma cells. This finding indicates that melanoma cells inhibit anti-tumor immunity via exosome secretion.
Visualization of exosomes is important to elucidate the role of exosomes in cell to cell comunication. Therefore, we constructed the expression vector of CD/GFP fusion protein using pEGFP-N1 vector. This vector makes us trace exosomes in vitro and in vivo.
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